Question (15767) | Anti-NMDAR1 C1 antibody (ab6484)

Go to datasheet (ab6484)

Question

I purchased this rabbit polyclonal AB against NMDAR1-C1 (ab6484) that works well for rat tissue but not at all for mouse. Has anyone else reported seeing this? My main question is about the AB immunogen. The protocol says that the AB was raised against the peptide representing the sequence of the C1 splice variant. Would you be able to tell me the exact peptide sequence used to make the AB? Did it cover the entire C1 cassette region or just a part of it. I'm very appreciative for any help you can give me.Thank you!

Answer

I have been informed that the immunogen used to generate ab6484 is the entire C1 splice variant. Please let me know if you have further questions,

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