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  1. Link

    nmdar1-phospho-s897-antibody-ab52184.pdf

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Neuroscience Neurotransmission Receptors / Channels Ligand-Gated Ion Channels NMDA Receptors
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Anti-NMDAR1 (phospho S897) antibody (ab52184)

  • Datasheet
Reviews (2) Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 (phospho S897) antibody (ab52184)
  • Western blot - Anti-NMDAR1 (phospho S897) antibody (ab52184)

Key features and details

  • Rabbit polyclonal to NMDAR1 (phospho S897)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human NMDAR1 protein (ab158584)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NMDAR1 (phospho S897) antibody
    See all NMDAR1 primary antibodies
  • Description

    Rabbit polyclonal to NMDAR1 (phospho S897)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human NMDAR1 aa 850-950 (phospho S897).
    Database link: Q05586

  • Positive control

    • Human brain tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • NMDA Receptors
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Corresponding non-phospho antibody

    • Anti-NMDAR1 antibody (ab52177)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human NMDAR1 protein (ab158584)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/1000. Predicted molecular weight: 105 kDa.
IHC-P
Use at an assay dependent concentration.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 105 kDa.
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
  • Post-translational
    modifications

    NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Target information above from: UniProt accession Q05586 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2902 Human
    • Entrez Gene: 14810 Mouse
    • Entrez Gene: 24408 Rat
    • Omim: 138249 Human
    • SwissProt: Q05586 Human
    • SwissProt: P35438 Mouse
    • SwissProt: P35439 Rat
    • Unigene: 558334 Human
    • Unigene: 278672 Mouse
    • Unigene: 9840 Rat
    see all
  • Alternative names

    • GluN1 antibody
    • Glutamate [NMDA] receptor subunit zeta-1 antibody
    • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
    • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
    • glutamate receptor ionotropic, NMDA 1 antibody
    • Grin1 antibody
    • MRD8 antibody
    • N methyl D aspartate receptor antibody
    • N methyl D aspartate receptor channel subunit zeta 1 antibody
    • N methyl D aspartate receptor subunit NR1 antibody
    • N-methyl-D-aspartate receptor subunit NR1 antibody
    • NMD-R1 antibody
    • NMDA 1 antibody
    • NMDA R1 antibody
    • NMDA receptor 1 antibody
    • NMDA1 antibody
    • NMDAR antibody
    • NMDZ1_HUMAN antibody
    • NR1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 (phospho S897) antibody (ab52184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 (phospho S897) antibody (ab52184)
    Ab52184 at 1/50 dilution staining Human brain tissue with and without blocking peptide; paraffin embedded.
  • Western blot - Anti-NMDAR1 (phospho S897) antibody (ab52184)
    Western blot - Anti-NMDAR1 (phospho S897) antibody (ab52184)

    Western Blot analysis of LOVO cell lysate labeling NMDAR1 (phospho S897) with ab52184. The lane on the right is blocked with the phospho peptide.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab52184? Please let us know so that we can cite the reference in this datasheet.

ab52184 has been referenced in 1 publication.

  • Lethbridge R  et al. Olfactory bulb glomerular NMDA receptors mediate olfactory nerve potentiation and odor preference learning in the neonate rat. PLoS One 7:e35024 (2012). IHC-FoFr ; Rat . PubMed: 22496886

Customer reviews and Q&As

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Western blot abreview for Anti-NMDAR1 (phospho S897) antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain, striatum)
Loading amount
20 µg
Specification
brain, striatum
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
SlimFast (soja-protein+milk) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 11 2011

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