Anti-NMDAR1 (phospho S897) antibody (ab52184)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-NMDAR1 (phospho S897) antibody
See all NMDAR1 primary antibodies -
DescriptionRabbit polyclonal to NMDAR1 (phospho S897)
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Host speciesRabbit
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SpecificityDetects endogenous levels of NMDAR1 only when phosphorylated at serine 897.
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Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic phosphopeptide derived from human NMDAR1 around the phosphorylation site of serine 897 (R-S-SP-K-D).
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Positive control
- Human brain tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Corresponding non-phospho antibody
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab52184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 105 kDa. | |
| ELISA | 1/10000. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
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Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
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Post-translational
modificationsNMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. -
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
- Information by UniProt
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Database links
- Entrez Gene: 2902 Human
- Entrez Gene: 14810 Mouse
- Entrez Gene: 24408 Rat
- Omim: 138249 Human
- SwissProt: Q05586 Human
- SwissProt: P35438 Mouse
- SwissProt: P35439 Rat
- Unigene: 558334 Human
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Alternative names
- GluN1 antibody
- Glutamate [NMDA] receptor subunit zeta-1 antibody
- Glutamate receptor ionotropic N methyl D aspartate 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 (phospho S897) antibody (ab52184)Ab52184 at 1/50 dilution staining Human brain tissue with and without blocking peptide; paraffin embedded. -
Western blot - Anti-NMDAR1 (phospho S897) antibody (ab52184)Western Blot analysis of LOVO cell lysate labeling NMDAR1 (phospho S897) with ab52184. The lane on the right is blocked with the phospho peptide.
Datasheets and documents
References
This product has been referenced in:
- Lethbridge R et al. Olfactory bulb glomerular NMDA receptors mediate olfactory nerve potentiation and odor preference learning in the neonate rat. PLoS One 7:e35024 (2012). IHC-FoFr ; Rat . Read more (PubMed: 22496886) »
