Product nameAnti-NMDAR2A antibody
See all NMDAR2A primary antibodies
DescriptionRabbit polyclonal to NMDAR2A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Monkey
Fusion protein (Mouse) (C terminal last 200 amino acids).
- WB: Whole cell lysate of synaptosomes of rat hippocampus.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 0.87% Sodium chloride
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab14596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 170 kDa.|
|IP||Use at an assay dependent dilution.|
FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EPND antibody
- FESD antibody
- GluN2A antibody
Anti-NMDAR2A antibody (ab14596) at 1/1000 dilution + whole cell lysate of synaptosomes of rat hippocampus at 15 µg
HRP-conjugated goat anti-rabbit antibody at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This product has been referenced in:
- McCarthny CR et al. Investigating the Interactive Effects of Sex Steroid Hormones and Brain-Derived Neurotrophic Factor during Adolescence on Hippocampal NMDA Receptor Expression. Int J Endocrinol 2018:7231915 (2018). Read more (PubMed: 29666640) »
- Notaras M et al. The BDNF Val66Met polymorphism regulates glucocorticoid-induced corticohippocampal remodeling and behavioral despair. Transl Psychiatry 7:e1233 (2017). WB ; Mouse . Read more (PubMed: 28926000) »