Overview

  • Product name
  • Description
    Rabbit polyclonal to NMDAR2A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Monkey
  • Immunogen

    Fusion protein (Mouse) (C terminal last 200 amino acids).

  • Positive control
    • WB: Whole cell lysate of synaptosomes of rat hippocampus.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.184% Tris glycine, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 170 kDa.
IP Use at an assay dependent dilution.

Target

  • Function
    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Images

  • Anti-NMDAR2A antibody (ab14596) at 1/1000 dilution + whole cell lysate of synaptosomes of rat hippocampus at 15 µg

    Secondary
    HRP-conjugated goat anti-rabbit antibody at 1/10000 dilution

    Developed using the ECL technique.

    Observed band size: 160 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

References

This product has been referenced in:
  • McCarthny CR  et al. Investigating the Interactive Effects of Sex Steroid Hormones and Brain-Derived Neurotrophic Factor during Adolescence on Hippocampal NMDA Receptor Expression. Int J Endocrinol 2018:7231915 (2018). Read more (PubMed: 29666640) »
  • Notaras M  et al. The BDNF Val66Met polymorphism regulates glucocorticoid-induced corticohippocampal remodeling and behavioral despair. Transl Psychiatry 7:e1233 (2017). WB ; Mouse . Read more (PubMed: 28926000) »
See all 21 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Chicken Cell lysate - whole cell (Homogenized chicken embryo cerebellum)
Gel Running Conditions
Non-reduced Denaturing (Non-reduced Denaturing (4-20% Mini-PROTEAN­ TGX™ Precast Gels))
Loading amount
25 µg
Specification
Homogenized chicken embryo cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mrs. Marthe Fjelldal

Verified customer

Submitted Mar 24 2017

Answer

Thank you for your message and for your patience.
It is difficult to find three secondary antibodies in three colors that woudl be suitable, but I hope the following will help. Please check the excitation and emission wavelenghts and spectra to ensure they are suitable for the machine you are using. (I say this because some of the blue dyes they suggest go into the green range in the light spectra). I can recommend to review the online datasheets for further information.
Anti NMDAR1 antibody (ab77264, goat polyclonal)
Red fluorophore Secondary antibodies for detection of goat primary:
Donkey anti-Goat IgG H&L (PE) secondary antibody (ab7004)
Donkey F(ab')2 polyclonal Secondary Antibody to Goat IgG - H&L (PE), pre-adsorbed
https://www.abcam.com/index.html?datasheet=7004 https://www.abcam.com/index.html?datasheet=7004.
Anti-NMDAR2A antibody (ab14596, rabbit polyclonal)
Green fluorophore Secondary antibodies for detection of rabbit primary:
ab6798 Donkey anti-Rabbit IgG H&L (FITC) secondary antibody
https://www.abcam.com/index.html?datasheet=6798 https://www.abcam.com/index.html?datasheet=6798.

ab7079 Donkey anti-Rabbit IgG H&L (FITC) secondary antibody
https://www.abcam.com/index.html?datasheet=7079 https://www.abcam.com/index.html?datasheet=7079.
Anti-NMDAR2B antibody (ab28373, mouse monoclonal)
The following may fit into the blue channel on the instrument you are using, but I can suggest to please check this. The emission spectra is slightly into the green spectra.
ab96875 Donkey anti-Mouse IgG H&L (DyLight® 488) secondary antibody
https://www.abcam.com/index.html?datasheet=96875 https://www.abcam.com/index.html?datasheet=96875.
I am sorry we do not have any that are tested and covered by the 6 month guarantee in western blot to suggest on this occasion.
The other option is to directly conjugate the primary antibodies yourself with our Easylink kits. These are quick and easy to use. However, please note that we recommend the following for these kits:
The antibody to be conjugated should be in 10-50mM amine-free buffer within a pH range of 6.5-8.5. Modest concentrations of Tris buffer (up to 20mM) are also tolerated.
I can recommend to read the following important information and frequently asked questions about the kits before selecting a suitable antibody to conjugate:
https://www.abcam.com/index.html?pageconfig=resource&rid=13148
https://www.abcam.com/index.html?pageconfig=resource&rid=13156
I hope this will be helpful. Please do not hesitate to contact me if you have any further questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - other (synaptosomes of rat hippocampus)
Loading amount
15 µg
Specification
synaptosomes of rat hippocampus
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Sophie Pezet

Verified customer

Submitted Apr 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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