Recombinant Anti-NMDAR2A antibody [EPR19694] (ab183939)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19694] to NMDAR2A
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-NMDAR2A antibody [EPR19694]
See all NMDAR2A primary antibodies -
Description
Rabbit monoclonal [EPR19694] to NMDAR2A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat hippocampus and brain lysates; His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19694 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 165 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 165 kDa). |
Target
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Function
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 14811 Mouse
- Entrez Gene: 24409 Rat
- SwissProt: P35436 Mouse
- SwissProt: Q00959 Rat
- Unigene: 2953 Mouse
- Unigene: 9710 Rat
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Alternative names
- EPND antibody
- FESD antibody
- GluN2A antibody
see all
Images
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All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/1000 dilution
Lane 1 : Mouse hippocampus lysate
Lane 2 : Rat hippocampus lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lane 5 : Mouse heart lysate
Lane 6 : Rat kidney lysate
Lane 7 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3 minutes; Lane 2: 30 seconds; Lane 3, 4, 5, 6 and 7: 3 minutes.
NMDAR2A has been previously documented to be specifically expressed in neuron cells. (PMID: 12127670).
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All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/5000 dilution
Lane 1 : His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326)
Lane 2 : His-tagged mouse NMDAR2B recombinant protein fragment (aa27-322)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 1 seconds; Lane 2: 3 minutes.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab183939 has been referenced in 1 publication.
- Giesen J et al. Hippocampal AMPA- and NMDA-induced cGMP signals are mainly generated by NO-GC2 and are under tight control by PDEs 1 and 2. Eur J Neurosci 55:18-31 (2022). PubMed: 34902209