Recombinant
RabMAb

Recombinant Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)

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Overview

  • Product name

    Anti-NMDAR2A antibody [EPR2465(2)]
    See all NMDAR2A primary antibodies

  • Description

    Rabbit monoclonal [EPR2465(2)] to NMDAR2A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NMDAR2A. The exact sequence is proprietary.

  • Positive control

    • WB: Mouse, rat and human brain tissue lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 7.10 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR2465(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 165 kDa.

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

Target

  • Function

    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.

  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.

  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Images

  • Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (purified) + Human cerebellum tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/20000 dilution (purified) + Human fetal brain tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (unpurified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Human brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 165 kDa

  • Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/2000 dilution

    Lane 1 : Human brain lysates prepared in RIPA lysis method
    Lane 2 : Human brain lysates prepared in 1%SDS Hot lysis method
    Lane 3 : Mouse brain lysates prepared in RIPA lysis method
    Lane 4 : Mouse brain lysates prepared in 1%SDS Hot lysis method
    Lane 5 : Rat brain lysates prepared in RIPA lysis method
    Lane 6 : Rat brain lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    Exposure time

    Lanes 1 to 2: 40 seconds
    Lanes 3 to 6: 20 seconds

    We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

    We are unsure how to define the extra bands.

  • Other - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Other - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Guo B  et al. Anterior cingulate cortex dysfunction underlies social deficits in Shank3 mutant mice. Nat Neurosci 22:1223-1234 (2019). Read more (PubMed: 31332372) »
  • Lin L  et al. Synaptic structure and alterations in the hippocampus in neonatal rats exposed to lipopolysaccharide. Neurosci Lett 709:134364 (2019). Read more (PubMed: 31288048) »
See all 16 Publications for this product

Publishing research using ab124913? Please let us know so that we can cite the reference in this datasheet.

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