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    nmdar2a-antibody-epr24652-ab124913.pdf

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Neuroscience Neurotransmission Receptors / Channels Ligand-Gated Ion Channels NMDA Receptors
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RecombinantRabMAb

Recombinant Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)

  • Datasheet
  • SDS
Submit a review Submit a question References (29)

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Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
  • Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
  • Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
  • Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
  • OI-RD Scanning - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
  • Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2465(2)] to NMDAR2A
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Carrier Free

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Overview

  • Product name

    Anti-NMDAR2A antibody [EPR2465(2)]
    See all NMDAR2A primary antibodies
  • Description

    Rabbit monoclonal [EPR2465(2)] to NMDAR2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NMDAR2A. The exact sequence is proprietary.

  • Positive control

    • WB: Mouse, rat and human brain tissue lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 7.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2465(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • NMDA Receptors
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-NMDAR2A antibody [EPR2465(2)] - BSA and Azide free (ab232332)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Rat brain normal tissue lysate - membrane extract (ab29473)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 165 kDa.

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

Notes
WB
1/1000. Predicted molecular weight: 165 kDa.

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

Target

  • Function

    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Target information above from: UniProt accession Q12879 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2903 Human
    • Entrez Gene: 14811 Mouse
    • Entrez Gene: 24409 Rat
    • Omim: 138253 Human
    • SwissProt: Q12879 Human
    • SwissProt: P35436 Mouse
    • SwissProt: Q00959 Rat
    • Unigene: 411472 Human
    • Unigene: 2953 Mouse
    • Unigene: 9710 Rat
    see all
  • Alternative names

    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Images

  • Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (purified) + Human cerebellum tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/20000 dilution (purified) + Human fetal brain tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (unpurified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Human brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 165 kDa

  • Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/2000 dilution

    Lane 1 : Human brain lysates prepared in RIPA lysis method
    Lane 2 : Human brain lysates prepared in 1%SDS Hot lysis method
    Lane 3 : Mouse brain lysates prepared in RIPA lysis method
    Lane 4 : Mouse brain lysates prepared in 1%SDS Hot lysis method
    Lane 5 : Rat brain lysates prepared in RIPA lysis method
    Lane 6 : Rat brain lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    Exposure time

    Lanes 1 to 2: 40 seconds
    Lanes 3 to 6: 20 seconds

    We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

    We are unsure how to define the extra bands.

  • OI-RD Scanning - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    OI-RD Scanning - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
    Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)

Protocols

  • Western blot protocols
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (29)

Publishing research using ab124913? Please let us know so that we can cite the reference in this datasheet.

ab124913 has been referenced in 29 publications.

  • Xu J  et al. Low-Dose Copper Exposure Exacerbates Depression-Like Behavior in ApoE4 Transgenic Mice. Oxid Med Cell Longev 2021:6634181 (2021). PubMed: 33833851
  • Loera-Valencia R  et al. High levels of 27-hydroxycholesterol results in synaptic plasticity alterations in the hippocampus. Sci Rep 11:3736 (2021). PubMed: 33580102
  • Borland JM  et al. Effect of Aggressive Experience in Female Syrian Hamsters on Glutamate Receptor Expression in the Nucleus Accumbens. Front Behav Neurosci 14:583395 (2020). PubMed: 33328919
  • Tyrtyshnaia A  et al. N-Docosahexanoylethanolamine Reduces Microglial Activation and Improves Hippocampal Plasticity in a Murine Model of Neuroinflammation. Int J Mol Sci 21:N/A (2020). PubMed: 33352646
  • Li Y  et al. Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins. Oxid Med Cell Longev 2020:5408452 (2020). PubMed: 32587661
View all Publications for this product

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