Recombinant Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
![Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)](https://www.abcam.com/ps/products/124/ab124913/Images/ab124913-243974-anti-nmdar2a-antibody-epr24652-western-blot.jpg "Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2465(2)] to NMDAR2A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-NMDAR2A antibody [EPR2465(2)]
See all NMDAR2A primary antibodies -
Description
Rabbit monoclonal [EPR2465(2)] to NMDAR2A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human NMDAR2A. The exact sequence is proprietary.
-
Positive control
- WB: Mouse, rat and human brain tissue lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 7.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2465(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 165 kDa.
We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 165 kDa. We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
Target
-
Function
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2903 Human
- Entrez Gene: 14811 Mouse
- Entrez Gene: 24409 Rat
- Omim: 138253 Human
- SwissProt: Q12879 Human
- SwissProt: P35436 Mouse
- SwissProt: Q00959 Rat
- Unigene: 411472 Human
see all -
Alternative names
- EPND antibody
- FESD antibody
- GluN2A antibody
see all
Images
-
Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (purified) + Human cerebellum tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
![Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)](https://www.abcam.com/ps/products/124/ab124913/Images/ab124913-243973-anti-nmdar2a-antibody-epr24652-western-blot.jpg)
Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/20000 dilution (purified) + Human fetal brain tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
![Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)](https://www.abcam.com/ps/products/124/ab124913/Images/ab124913-138907-anti-nmdar2a-antibody-epr24652-western-blot.jpg)
Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (unpurified)
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 165 kDa -
![Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)](https://www.abcam.com/ps/products/124/ab124913/Images/ab124913-318102-anti-nmdar2a-antibody-epr24652-western-blot.jpg)
Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/2000 dilution
Lane 1 : Human brain lysates prepared in RIPA lysis method
Lane 2 : Human brain lysates prepared in 1%SDS Hot lysis method
Lane 3 : Mouse brain lysates prepared in RIPA lysis method
Lane 4 : Mouse brain lysates prepared in 1%SDS Hot lysis method
Lane 5 : Rat brain lysates prepared in RIPA lysis method
Lane 6 : Rat brain lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 165 kDa
Observed band size: 165 kDaExposure time
Lanes 1 to 2: 40 seconds
Lanes 3 to 6: 20 secondsWe recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
We are unsure how to define the extra bands.
-
![OI-RD Scanning - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)](https://www.abcam.com/ps/products/124/ab124913/Images/ab124913-138906-anti-nmdar2a-antibody-epr24652.jpg)
OI-RD Scanning - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) -
![Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)](https://www.abcam.com/ps/products/124/ab124913/Images/ab124913-7-benefits-of-recombinant-antibodies.png)
Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
Datasheets and documents
-
SDS download
-
Datasheet download
References (29)
ab124913 has been referenced in 29 publications.
- Xu J et al. Low-Dose Copper Exposure Exacerbates Depression-Like Behavior in ApoE4 Transgenic Mice. Oxid Med Cell Longev 2021:6634181 (2021). PubMed: 33833851
- Loera-Valencia R et al. High levels of 27-hydroxycholesterol results in synaptic plasticity alterations in the hippocampus. Sci Rep 11:3736 (2021). PubMed: 33580102
- Borland JM et al. Effect of Aggressive Experience in Female Syrian Hamsters on Glutamate Receptor Expression in the Nucleus Accumbens. Front Behav Neurosci 14:583395 (2020). PubMed: 33328919
- Tyrtyshnaia A et al. N-Docosahexanoylethanolamine Reduces Microglial Activation and Improves Hippocampal Plasticity in a Murine Model of Neuroinflammation. Int J Mol Sci 21:N/A (2020). PubMed: 33352646
- Li Y et al. Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins. Oxid Med Cell Longev 2020:5408452 (2020). PubMed: 32587661