Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-NMDAR2A antibody [EPR2465(2)]
See all NMDAR2A primary antibodies -
DescriptionRabbit monoclonal [EPR2465(2)] to NMDAR2A
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human NMDAR2A. The exact sequence is proprietary.
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Positive control
- WB: Mouse, rat and human brain tissue lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Dissociation constant (KD)KD = 7.10 x 10 -11 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR2465(2)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 165 kDa. We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
Target
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FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
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Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
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Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 2903 Human
- Entrez Gene: 14811 Mouse
- Entrez Gene: 24409 Rat
- Omim: 138253 Human
- SwissProt: Q12879 Human
- SwissProt: P35436 Mouse
- SwissProt: Q00959 Rat
- Unigene: 411472 Human
see all -
Alternative names
- EPND antibody
- FESD antibody
- GluN2A antibody
see all
Images
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![Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)](https://www.abcam.com/ps/products/124/ab124913/Images/ab124913-4-ab124913WB2.jpg)
Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (purified) + Human cerebellum tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)](https://www.abcam.com/ps/products/124/ab124913/Images/ab124913-3-ab124913WB1.jpg)
Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/20000 dilution (purified) + Human fetal brain tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)](https://www.abcam.com/ps/products/124/ab124913/Images/NMDAR2A-Primary-antibodies-ab124913-2.jpg)
Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (unpurified)
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 165 kDa -
![Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)](https://www.abcam.com/ps/products/124/ab124913/Images/ab124913-318102-ab124913.jpg)
Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/2000 dilution
Lane 1 : Human brain lysates prepared in RIPA lysis method
Lane 2 : Human brain lysates prepared in 1%SDS Hot lysis method
Lane 3 : Mouse brain lysates prepared in RIPA lysis method
Lane 4 : Mouse brain lysates prepared in 1%SDS Hot lysis method
Lane 5 : Rat brain lysates prepared in RIPA lysis method
Lane 6 : Rat brain lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 165 kDa
Observed band size: 165 kDaExposure time
Lanes 1 to 2: 40 seconds
Lanes 3 to 6: 20 secondsWe recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
We are unsure how to define the extra bands.
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![Other - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)](https://www.abcam.com/ps/products/124/ab124913/Images/NMDAR2A-Primary-antibodies-ab124913-3.jpg)
Other - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
Protocols
References
This product has been referenced in:
- Zhuang W et al. Berberine exerts antioxidant effects via protection of spiral ganglion cells against cytomegalovirus-induced apoptosis. Free Radic Biol Med 121:127-135 (2018). Read more (PubMed: 29715550) »
- Marschner L et al. Single mild traumatic brain injury results in transiently impaired spatial long-term memory and altered search strategies. Behav Brain Res N/A:N/A (2018). WB ; Mouse . Read more (PubMed: 29499284) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"