Recombinant Anti-NMDAR2A antibody [EPR7063] (ab133265)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7063] to NMDAR2A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NMDAR2A antibody [EPR7063]
See all NMDAR2A primary antibodies -
Description
Rabbit monoclonal [EPR7063] to NMDAR2A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Mouse brain lysate, Rat brain lysate, Human hypothalamus lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7063 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 165 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 165 kDa). |
Target
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Function
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2903 Human
- Entrez Gene: 14811 Mouse
- Entrez Gene: 24409 Rat
- Omim: 138253 Human
- SwissProt: Q12879 Human
- SwissProt: P35436 Mouse
- SwissProt: Q00959 Rat
- Unigene: 411472 Human
see all -
Alternative names
- EPND antibody
- FESD antibody
- GluN2A antibody
see all
Images
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All lanes : Anti-NMDAR2A antibody [EPR7063] (ab133265) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 165 kDa -
Anti-NMDAR2A antibody [EPR7063] (ab133265) at 1/1000 dilution + Human hypothalamus lysate at 10 µg
Secondary
Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 165 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab133265 has been referenced in 17 publications.
- Li R et al. Sevoflurane Exposure in the Developing Brain Induces Hyperactivity, Anxiety-Free, and Enhancement of Memory Consolidation in Mice. Front Aging Neurosci 14:934230 (2022). PubMed: 35847668
- Cao W et al. NMDA receptor hypofunction underlies deficits in parvalbumin interneurons and social behavior in neuroligin 3 R451C knockin mice. Cell Rep 41:111771 (2022). PubMed: 36476879
- Xu L et al. Deficits in N-Methyl-D-Aspartate Receptor Function and Synaptic Plasticity in Hippocampal CA1 in APP/PS1 Mouse Model of Alzheimer's Disease. Front Aging Neurosci 13:772980 (2021). PubMed: 34916926
- Rosendo-Pineda MJ et al. Phosphorylation of NMDA receptors by cyclin B/CDK1 modulates calcium dynamics and mitosis. Commun Biol 3:665 (2020). PubMed: 33184446
- Pegasiou CM et al. Age-Dependent Changes in Synaptic NMDA Receptor Composition in Adult Human Cortical Neurons. Cereb Cortex 30:4246-4256 (2020). PubMed: 32191258