Overview

  • Product name

    Anti-NMDAR2A antibody [EPR7063]
    See all NMDAR2A primary antibodies
  • Description

    Rabbit monoclonal [EPR7063] to NMDAR2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within Human NMDAR2A (Q12879).

  • Positive control

    • Mouse brain lysate, Rat brain lysate, Human hypothalamus lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 165 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
    • Sequence similarities

      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
    • Cellular localization

      Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • EPND antibody
      • FESD antibody
      • GluN2A antibody
      • Glutamate [NMDA] receptor subunit epsilon-1 antibody
      • Glutamate receptor antibody
      • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
      • GRIN 2A antibody
      • GRIN2A antibody
      • hNR2A antibody
      • LKS antibody
      • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
      • N Methyl D Aspartate Receptor Subtype 2A antibody
      • N methyl D aspartate receptor subunit 2A antibody
      • N-methyl D-aspartate receptor subtype 2A antibody
      • NMDA receptor subtype 2A antibody
      • NMDAR 2A antibody
      • NMDAR2A antibody
      • NMDE1_HUMAN antibody
      • NR2A antibody
      • OTTHUMP00000160135 antibody
      • OTTHUMP00000174531 antibody
      see all

    Images

    • All lanes : Anti-NMDAR2A antibody [EPR7063] (ab133265) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 165 kDa

    • Anti-NMDAR2A antibody [EPR7063] (ab133265) at 1/1000 dilution + Human hypothalamus lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 165 kDa

    References

    This product has been referenced in:

    • Gong WY  et al. Chronic Monoarthritis Pain Accelerates the Processes of Cognitive Impairment and Increases the NMDAR Subunits NR2B in CA3 of Hippocampus from 5-month-old Transgenic APP/PS1 Mice. Front Aging Neurosci 9:123 (2017). Read more (PubMed: 28553223) »
    • Zhou X  et al. Differential Modulation of GABAAand NMDA Receptors by an a7-nicotinic Acetylcholine Receptor Agonist in Chronic Glaucoma. Front Mol Neurosci 10:422 (2017). Read more (PubMed: 29326549) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    Question
    Answer

    We have indeed tested ab133265 in IP however, in our hands this product did not give good results in this application and therefore we have concluded that it is unsuitable for use in IP.

    If you are looking for an antibody guaranteed to work in IP, I could suggest the following:

    https://www.abcam.com/nmdar2a-antibody-ab14596.html guaranteed to work in IP in Mouse, Rat, Monkey
    https://www.abcam.com/nmdar2a-antibody-ab169873.html guaranteed to work in IP in Mouse, Rat, Human

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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