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Fusion protein (His-tag) corresponding to Rat NMDAR2A aa 75-325 (N terminal).
NRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPI LGIHGGASMI MADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFI SFIKTTVDNS FVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEAR SLGLTGYDFF WIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAAS SMLEKFSYIP EAKASCYGQAE
The clone number has been updated from S327A-38 to N327A/38, both clone numbers name the same antibody clone.
Our Abpromise guarantee covers the use of ab174636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 165 kDa.|
4% formaldehyde-fixed SK-N-BE cells labeling NMDAR2A using ab174636 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/200 dilution incubated for 1 hour at room temperature. Counter stained with Phalloidin Texas Red F-actin stain and DAPI (blue) nuclear stain.
ab174636 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"