Product nameAnti-NMDAR2A antibody [N327A/38] - N-terminal
See all NMDAR2A primary antibodies
DescriptionMouse monoclonal [N327A/38] to NMDAR2A - N-terminal
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein (His-tag) corresponding to Rat NMDAR2A aa 75-325 (N terminal).
NRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPI LGIHGGASMI MADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFI SFIKTTVDNS FVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEAR SLGLTGYDFF WIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAAS SMLEKFSYIP EAKASCYGQAE
Database link: Q00959
- COS cells transiently transfected with GFP-tagged NMDAR2A lysate. ICC/IF: SK-N-BE cells.
The clone number has been updated from S327A-38 to N327A/38, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab174636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 165 kDa.|
FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EPND antibody
- FESD antibody
- GluN2A antibody
4% formaldehyde-fixed SK-N-BE cells labeling NMDAR2A using ab174636 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/200 dilution incubated for 1 hour at room temperature. Counter stained with Phalloidin Texas Red F-actin stain and DAPI (blue) nuclear stain.
Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636) at 1/200 dilution + Monkey COS cells transfected with GFP-tagged NMDAR2A at 15 µg with Monkey COS cells transfected with GFP-tagged NR2A
Goat Anti-Mouse IgG: HRP for 1 hour RT. at 1/1000 dilution
Predicted band size: 165 kDa
Observed band size: ~170 kDa why is the actual band size different from the predicted?
Color Development: ECL solution for 6 min in RT.
ab174636 has not yet been referenced specifically in any publications.