Key features and details
- Mouse monoclonal [N327A/38] to NMDAR2A - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Product nameAnti-NMDAR2A antibody [N327A/38] - N-terminal
See all NMDAR2A primary antibodies
DescriptionMouse monoclonal [N327A/38] to NMDAR2A - N-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein (His-tag) corresponding to Rat NMDAR2A aa 75-325 (N terminal).
NRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPI LGIHGGASMI MADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFI SFIKTTVDNS FVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEAR SLGLTGYDFF WIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAAS SMLEKFSYIP EAKASCYGQAE
Database link: Q00959
- COS cells transiently transfected with GFP-tagged NMDAR2A lysate. ICC/IF: SK-N-BE cells.
The clone number has been updated from S327A-38 to N327A/38, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab174636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 165 kDa.|
FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EPND antibody
- FESD antibody
- GluN2A antibody
4% formaldehyde-fixed SK-N-BE cells labeling NMDAR2A using ab174636 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/200 dilution incubated for 1 hour at room temperature. Counter stained with Phalloidin Texas Red F-actin stain and DAPI (blue) nuclear stain.
Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636) at 1/200 dilution + Monkey COS cells transfected with GFP-tagged NMDAR2A at 15 µg with Monkey COS cells transfected with GFP-tagged NR2A
Goat Anti-Mouse IgG: HRP for 1 hour RT. at 1/1000 dilution
Predicted band size: 165 kDa
Observed band size: ~170 kDa why is the actual band size different from the predicted?
Color Development: ECL solution for 6 min in RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab174636 has not yet been referenced specifically in any publications.