Product nameAnti-NMDAR2A antibody [S327-95] - N-terminal
See all NMDAR2A primary antibodies
DescriptionMouse monoclonal [S327-95] to NMDAR2A - N-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee
Fusion protein corresponding to Rat NMDAR2A aa 75-325. (extracellular N-terminus) NP_036705.3.
NRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPI LGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVT TIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHS SVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPS GLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQA E
Database link: Q00959
- WB: Monkey COS cells transfected with GFP-tagged NMDAR2A. ICC/IF: SK-N-BE cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab240884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EPND antibody
- FESD antibody
- GluN2A antibody
Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (human neuroblastoma cell line) cells labeling NMDAR2A using ab240884 at 1/100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at RT.
Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min RT, 5 min RT.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240884 (D) Composite.
Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884) at 1/200 dilution (16 hours at 4°C) + Monkey COS cells transfected with GFP-tagged NMDAR2A at 15 µg
Goat Anti-Mouse IgG: HRP for 1 hour RT at 1/1000 dilution
Developed using the ECL technique.
Exposure time: 6 minutes
Block: 2% BSA and 2% skimmed milk in 1X TBST.
ab240884 has not yet been referenced specifically in any publications.