Key features and details
- Mouse monoclonal [S327-95] to NMDAR2A - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-NMDAR2A antibody [S327-95] - N-terminal
See all NMDAR2A primary antibodies
DescriptionMouse monoclonal [S327-95] to NMDAR2A - N-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee
Fusion protein corresponding to Rat NMDAR2A aa 75-325. (extracellular N-terminus) NP_036705.3.
NRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPI LGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVT TIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHS SVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPS GLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQA E
Database link: Q00959
- WB: Monkey COS cells transfected with GFP-tagged NMDAR2A. ICC/IF: SK-N-BE cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab240884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EPND antibody
- FESD antibody
- GluN2A antibody
Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (human neuroblastoma cell line) cells labeling NMDAR2A using ab240884 at 1/100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at RT.
Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min RT, 5 min RT.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240884 (D) Composite.
Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884) at 1/200 dilution (16 hours at 4°C) + Monkey COS cells transfected with GFP-tagged NMDAR2A at 15 µg
Goat Anti-Mouse IgG: HRP for 1 hour RT at 1/1000 dilution
Developed using the ECL technique.
Exposure time: 6 minutes
Block: 2% BSA and 2% skimmed milk in 1X TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240884 has not yet been referenced specifically in any publications.