Overview

  • Product name

    Anti-NMDAR2A (phospho Y1325) antibody
    See all NMDAR2A primary antibodies
  • Description

    Rabbit polyclonal to NMDAR2A (phospho Y1325)
  • Host species

    Rabbit
  • Specificity

    Specific for the ~180k NMDAR2A protein phosphorylated at Tyr1325 in Western blots. Immunolabelling is completely blocked by preadsorption with the phosphopeptide but not by the corresponding dephosphopeptide.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Dog
  • Immunogen

    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of NMDAR2A.

  • Positive control

    • Rat hippocampal lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.50
    Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 165 kDa.

Target

  • Function

    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Images

  • All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/1000 dilution

    Lane 1 : Rat hippocampal lysate
    Lane 2 : Rat hippocampal lysate with preadsorption with the phospho-peptide

    Predicted band size: 165 kDa
    Observed band size: ~180 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/500 dilution

    All lanes : Rat brain whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 165 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

References

This product has been referenced in:

  • Go BS  et al. Glutamatergic neurotransmission in the prefrontal cortex mediates the suppressive effect of intra-prelimbic cortical infusion of BDNF on cocaine-seeking. Eur Neuropsychopharmacol 26:1989-1999 (2016). Read more (PubMed: 27765467) »
  • Taniguchi S  et al. Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour. EMBO J 28:3717-29 (2009). Read more (PubMed: 19834457) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Loading amount
20 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (3-8% Acetate)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Xiaofan Wang

Verified customer

Submitted Apr 27 2012

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