Key features and details
- Rabbit polyclonal to NMDAR2A (phospho Y1325)
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-NMDAR2A (phospho Y1325) antibody
See all NMDAR2A primary antibodies
DescriptionRabbit polyclonal to NMDAR2A (phospho Y1325)
SpecificitySpecific for the ~180k NMDAR2A protein phosphorylated at Tyr1325 in Western blots. Immunolabelling is completely blocked by preadsorption with the phosphopeptide but not by the corresponding dephosphopeptide.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Cow, Dog
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of NMDAR2A.
- Rat hippocampal lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES
Concentration information loading...
PurityProtein A purified
Purification notesPrepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Our Abpromise guarantee covers the use of ab106590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 165 kDa.|
FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
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All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate with preadsorption with the phospho-peptide
Predicted band size: 165 kDa
Observed band size: ~180 kDa why is the actual band size different from the predicted?
All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/500 dilution
All lanes : Rat brain whole tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 165 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab106590 has been referenced in 2 publications.
- Go BS et al. Glutamatergic neurotransmission in the prefrontal cortex mediates the suppressive effect of intra-prelimbic cortical infusion of BDNF on cocaine-seeking. Eur Neuropsychopharmacol 26:1989-1999 (2016). PubMed: 27765467
- Taniguchi S et al. Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour. EMBO J 28:3717-29 (2009). PubMed: 19834457