Product nameAnti-NMDAR2A (phospho Y1325) antibody
See all NMDAR2A primary antibodies
DescriptionRabbit polyclonal to NMDAR2A (phospho Y1325)
Tested applicationsSuitable for: IHC-P, IHC-FoFr, WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Synthetic peptide corresponding to Mouse NMDAR2A aa 1300-1400 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Mouse brain
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab16646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa).|
FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EPND antibody
- FESD antibody
- GluN2A antibody
All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab16646) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate with Mouse NMDAR2A (phospho Y1325) peptide (ab16985) at 1 µg/ml
Lane 3 : Brain (Mouse) Tissue Lysate with Mouse NMDAR2A peptide (ab16990) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 163 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 260 kDa, 400 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Immunohistochemistical detection of NMDAR2A (phospho Y1325) using antibody ab16646 on paraformaldehyde perfusion fixed rat brain sections. Primary Antibody was used at 1/300, incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: Goat anti-rabbit Alexa Fluor 488 (1/1000). The antibody produced a diffuse staining in the whole CNS; some cellular processes are stained too.
This product has been referenced in:
- Bartosz P et al. Benzophenone-3 passes through the blood-brain barrier, increases the level of extracellular glutamate and induces apoptotic processes in the hippocampus and frontal cortex of rats. Toxicol Sci N/A:N/A (2019). Read more (PubMed: 31368502) »
- Birla H et al. Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice. Behav Brain Funct 15:9 (2019). Read more (PubMed: 31064381) »