Anti-NMDAR2A (phospho Y1325) antibody (ab16646)
Key features and details
- Rabbit polyclonal to NMDAR2A (phospho Y1325)
- Suitable for: IHC-P, WB, IHC-FoFr
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
-
Product name
Anti-NMDAR2A (phospho Y1325) antibody
See all NMDAR2A primary antibodies -
Description
Rabbit polyclonal to NMDAR2A (phospho Y1325) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
-
Immunogen
Synthetic peptide corresponding to Mouse NMDAR2A aa 1300-1400 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab16985) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa).
|
|
| IHC-FoFr |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa). |
|
IHC-FoFr
Use at an assay dependent concentration. |
Target
-
Function
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2903 Human
- Entrez Gene: 14811 Mouse
- Entrez Gene: 24409 Rat
- Omim: 138253 Human
- SwissProt: Q12879 Human
- SwissProt: P35436 Mouse
- SwissProt: Q00959 Rat
- Unigene: 411472 Human
see all -
Alternative names
- EPND antibody
- FESD antibody
- GluN2A antibody
see all
Images
-
Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab16646)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
-
Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab16646)All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab16646) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate with Mouse NMDAR2A (phospho Y1325) peptide (ab16985) at 1 µg/ml
Lane 3 : Brain (Mouse) Tissue Lysate with Mouse NMDAR2A peptide (ab16990) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 163 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 260 kDa, 400 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Datasheets and documents
-
SDS download
-
Datasheet download
References (17)
ab16646 has been referenced in 17 publications.
- Tan Y et al. LY354740 Reduces Extracellular Glutamate Concentration, Inhibits Phosphorylation of Fyn/NMDARs, and Expression of PLK2/pS129 a-Synuclein in Mice Treated With Acute or Sub-Acute MPTP. Front Pharmacol 11:183 (2020). PubMed: 32180729
- Liang L et al. Involvement of homodomain interacting protein kinase 2-c-Jun N-terminal kinase/c-Jun cascade in the long-term synaptic toxicity and cognition impairment induced by neonatal Sevoflurane exposure. J Neurochem 154:372-388 (2020). PubMed: 31705656
- Zhuravin IA et al. Prenatal hypoxia produces memory deficits associated with impairment of long-term synaptic plasticity in young rats. Neurobiol Learn Mem 164:107066 (2019). PubMed: 31400467
- Gisslen T et al. Fetal inflammation is associated with persistent systemic and hippocampal inflammation and dysregulation of hippocampal glutamatergic homeostasis. Pediatr Res 85:703-710 (2019). PubMed: 30745569
- Bartosz P et al. Benzophenone-3 passes through the blood-brain barrier, increases the level of extracellular glutamate and induces apoptotic processes in the hippocampus and frontal cortex of rats. Toxicol Sci N/A:N/A (2019). PubMed: 31368502