Anti-NMDAR2A (phospho Y1325) antibody (ab16646)
- Datasheet
- References (10)
- Protocols
Overview
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Product nameAnti-NMDAR2A (phospho Y1325) antibody
See all NMDAR2A primary antibodies -
DescriptionRabbit polyclonal to NMDAR2A (phospho Y1325)
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, IHC-FoFr, WBmore details
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Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1300 - 1400 of Mouse NMDAR2A, phosphorylated at Y1325.
Read Abcam's proprietary immunogen policy (Peptide available as ab16985.) -
Positive control
- Mouse brain
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab16646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| IHC-FoFr | 1/300. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa). |
Target
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FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
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Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
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Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 2903 Human
- Entrez Gene: 14811 Mouse
- Entrez Gene: 24409 Rat
- Omim: 138253 Human
- SwissProt: Q12879 Human
- SwissProt: P35436 Mouse
- SwissProt: Q00959 Rat
- Unigene: 411472 Human
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Alternative names
- EPND antibody
- FESD antibody
- GluN2A antibody
see all
Images
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Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab16646)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom -
Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab16646)All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab16646) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate with Mouse NMDAR2A (phospho Y1325) peptide (ab16985) at 1 µg/ml
Lane 3 : Brain (Mouse) Tissue Lysate with Mouse NMDAR2A peptide (ab16990) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 163 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 260 kDa, 400 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NMDAR2A (phospho Y1325) antibody (ab16646)This image is courtesy of an abreview submitted by Sophie Pezet, CNRS, Paris, FranceImmunohistochemistical detection of NMDAR2A (phospho Y1325) using antibody ab16646 on paraformaldehyde perfusion fixed rat brain sections. Primary Antibody was used at 1/300, incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: Goat anti-rabbit Alexa Fluor 488 (1/1000). The antibody produced a diffuse staining in the whole CNS; some cellular processes are stained too.
References
This product has been referenced in:
- Kim J et al. Regulator of G-Protein Signaling 4 (RGS4) Controls Morphine Reward by Glutamate Receptor Activation in the Nucleus Accumbens of Mouse Brain. Mol Cells 41:454-464 (2018). Read more (PubMed: 29754475) »
- Rodríguez-Muñoz M et al. Fenfluramine diminishes NMDA receptor-mediated seizures via its mixed activity at serotonin 5HT2A and type 1 sigma receptors. Oncotarget 9:23373-23389 (2018). Read more (PubMed: 29805740) »
