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Neuroscience Neurotransmission Receptors / Channels Ligand-Gated Ion Channels NMDA Receptors
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Anti-NMDAR2A (phospho Y1325) antibody (ab16646)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (14)

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Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab16646)
  • Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab16646)

Key features and details

  • Rabbit polyclonal to NMDAR2A (phospho Y1325)
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NMDAR2A (phospho Y1325) antibody
    See all NMDAR2A primary antibodies
  • Description

    Rabbit polyclonal to NMDAR2A (phospho Y1325)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse NMDAR2A aa 1300-1400 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab16985)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • NMDA Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
All applications
Rat
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa).

Target

  • Function

    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Target information above from: UniProt accession Q12879 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2903 Human
    • Entrez Gene: 14811 Mouse
    • Entrez Gene: 24409 Rat
    • Omim: 138253 Human
    • SwissProt: Q12879 Human
    • SwissProt: P35436 Mouse
    • SwissProt: Q00959 Rat
    • Unigene: 411472 Human
    • Unigene: 2953 Mouse
    • Unigene: 9710 Rat
    see all
  • Alternative names

    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Images

  • Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab16646)
    Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab16646)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
  • Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab16646)
    Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab16646)
    All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab16646) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate with Mouse NMDAR2A (phospho Y1325) peptide (ab16985) at 1 µg/ml
    Lane 3 : Brain (Mouse) Tissue Lysate with Mouse NMDAR2A peptide (ab16990) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 163 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 260 kDa, 400 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (14)

    Publishing research using ab16646? Please let us know so that we can cite the reference in this datasheet.

    ab16646 has been referenced in 14 publications.

    • Tan Y  et al. LY354740 Reduces Extracellular Glutamate Concentration, Inhibits Phosphorylation of Fyn/NMDARs, and Expression of PLK2/pS129 a-Synuclein in Mice Treated With Acute or Sub-Acute MPTP. Front Pharmacol 11:183 (2020). PubMed: 32180729
    • Gisslen T  et al. Fetal inflammation is associated with persistent systemic and hippocampal inflammation and dysregulation of hippocampal glutamatergic homeostasis. Pediatr Res 85:703-710 (2019). PubMed: 30745569
    • Bartosz P  et al. Benzophenone-3 passes through the blood-brain barrier, increases the level of extracellular glutamate and induces apoptotic processes in the hippocampus and frontal cortex of rats. Toxicol Sci N/A:N/A (2019). PubMed: 31368502
    • Birla H  et al. Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice. Behav Brain Funct 15:9 (2019). PubMed: 31064381
    • Hasegawa S  et al. Acute administration of ketamine attenuates the impairment of social behaviors induced by social defeat stress exposure as juveniles via activation of a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors. Neuropharmacology 148:107-116 (2018). PubMed: 30590060
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NMDAR2A (phospho Y1325) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain sections)
    Specification
    Brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Read More

    Dr. Sophie Pezet

    Verified customer

    Submitted Mar 22 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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