Key features and details
- Rabbit polyclonal to NMDAR2B
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-NMDAR2B antibody
See all NMDAR2B primary antibodies
DescriptionRabbit polyclonal to NMDAR2B
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
Synthetic peptide within Human NMDAR2B aa 465-515 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q13224
- Rat brain tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.
(or 1/50 - 1/200 if using a fluorescent secondary antibody).
FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Tissue specificityPrimarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling NMDAR2B with ab216621 at 1/300 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling NMDAR2B with ab216621 at 1/200 dilution, followed by Goat anti-Rabbit IgG, FITC conjugate at 1/200 dilution for 40 minutes at 37°C and DAPI staining.
ab216621 has been referenced in 1 publication.
- Zhao L et al. Effects of intrathecal bupivacaine on the NR2B/CaMKIIa/CREB signaling pathway in the rat lumbar spinal cord. Mol Med Rep 17:4508-4514 (2018). PubMed: 29344649