Western blot abreview for Anti-NMDAR2B antibody

Excellent
Abreviews
Application
Western blot
Loading amount
2 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Xenopus laevis Cell lysate - whole cell (Tectal cell)
Specification
Tectal cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS in 0.1% Tween20
Dilution
1/300

Secondary antibody

Name
Non-Abcam antibody was used: Bio-Rad Goat anti-Rabbit IgG HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Bands
Specific: 180 kDa Non-specific: 105, 70, 40, 30 kDa
Exposure
10 second(s)
Positive control
MS:Whole brain lysates from mouse cortex XL: Whole brain lysated from Xenopus Laevis tectum

Additional data

Additional Notes
I attended the Abtrial program.

Dr. Wanhua Shen

Verified customer

Submitted Aug 01 2014

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