Key features and details
- Rabbit polyclonal to NMDAR2B
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-NMDAR2B antibody
See all NMDAR2B primary antibodies
DescriptionRabbit polyclonal to NMDAR2B
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Chicken, Cow, Dog, Human, Non human primates
Synthetic peptide from the N-terminus of rat NMDAR2B.
- Rat hippocampal lysate.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab73001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).|
FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Tissue specificityPrimarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
Anti-NMDAR2B antibody (ab73001) at 1/1000 dilution + rat hippocampal lysate at 10 µg
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: <68 kDa, 120 kDa. We are unsure as to the identity of these extra bands.
ab73001 diluted 1/500 staining NMDAR2B on 14DIV cultured rat cortical neurons fixed in methanol at - 20oC for 10 minutes. Sections were also labelled with an anti-PSD95 antibody in red.
ab73001 has been referenced in 3 publications.
- Song Y et al. A novel mechanism of synaptic and cognitive impairments mediated via microRNA-30b in Alzheimer's disease. EBioMedicine N/A:N/A (2018). PubMed: 30522932
- Zhang J & Chen C Alleviation of Neuropathology by Inhibition of Monoacylglycerol Lipase in APP Transgenic Mice Lacking CB2 Receptors. Mol Neurobiol 55:4802-4810 (2018). PubMed: 28733897
- Zhang J et al. Synaptic and cognitive improvements by inhibition of 2-AG metabolism are through upregulation of microRNA-188-3p in a mouse model of Alzheimer's disease. J Neurosci 34:14919-33 (2014). WB ; Mouse . PubMed: 25378159