Key features and details
- Rabbit polyclonal to NMDAR2B - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-NMDAR2B antibody - C-terminal
See all NMDAR2B primary antibodies
DescriptionRabbit polyclonal to NMDAR2B - C-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Cow, Dog, Human
Synthetic peptide within Human NMDAR2B (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: Q13224
- ICC/IF: DIV10 rat E18 primary hippocampal neurons. IHC-P: Rat brain tissue. WB: Mouse hippocampus tissue extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 166 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Tissue specificityPrimarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
Anti-NMDAR2B antibody - C-terminal (ab229636) at 1/1000 dilution + Mouse hippocampus tissue extract at 50 µg
Developed using the ECL technique.
Predicted band size: 166 kDa
DIV10 rat E18 primary hippocampal neurons stained for NMDAR2B (green) using ab229636 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue) and beta Tubulin 3/ Tuj1 (red).
Paraffin-embedded rat brain tissue stained for NMDAR2B with ab229636 at a 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229636 has not yet been referenced specifically in any publications.