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  1. Link

    nmdar2b-antibody-epr19295-ab183942.pdf

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Neuroscience Neurotransmission Receptors / Channels Ligand-Gated Ion Channels NMDA Receptors
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RecombinantRabMAb

Recombinant Anti-NMDAR2B antibody [EPR19295] (ab183942)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (2)

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Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)
  • Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)
  • Anti-NMDAR2B antibody [EPR19295] (ab183942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19295] to NMDAR2B
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
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Recombinant Human NMDAR2B protein (ab112298)
Primary
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Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NMDAR2B antibody [EPR19295]
    See all NMDAR2B primary antibodies
  • Description

    Rabbit monoclonal [EPR19295] to NMDAR2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse NMDAR2B aa 1450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q01097

  • Positive control

    • WB: Human hippocampus lysate; Mouse brain and hippocampus lysates; Rat brain and hippocampus lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19295
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • NMDA Receptors
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other

Associated products

  • Alternative Versions

    • Anti-NMDAR2B antibody [EPR19295] - BSA and Azide free (ab250711)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human NMDAR2B protein (ab112298)

Applications

Our Abpromise guarantee covers the use of ab183942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).

Target

  • Function

    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
  • Tissue specificity

    Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Target information above from: UniProt accession Q13224 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2904 Human
    • Entrez Gene: 14812 Mouse
    • Entrez Gene: 24410 Rat
    • Omim: 138252 Human
    • SwissProt: Q13224 Human
    • SwissProt: Q01097 Mouse
    • SwissProt: Q00960 Rat
    • Unigene: 654430 Human
    • Unigene: 436649 Mouse
    • Unigene: 9711 Rat
    see all
  • Alternative names

    • AW490526 antibody
    • EIEE27 antibody
    • Glutamate [NMDA] receptor subunit epsilon 2 antibody
    • Glutamate [NMDA] receptor subunit epsilon-2 antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
    • Glutamate receptor ionotropic NMDA2B antibody
    • Glutamate receptor subunit epsilon 2 antibody
    • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
    • GRIN 2B antibody
    • GRIN2B antibody
    • hNR 3 antibody
    • hNR3 antibody
    • MGC142178 antibody
    • MGC142180 antibody
    • MRD6 antibody
    • N methyl D asparate receptor channel subunit epsilon 2 antibody
    • N methyl D aspartate receptor subtype 2B antibody
    • N methyl D aspartate receptor subunit 2B antibody
    • N methyl D aspartate receptor subunit 3 antibody
    • N-methyl D-aspartate receptor subtype 2B antibody
    • N-methyl-D-aspartate receptor subunit 3 antibody
    • NMDA NR2B antibody
    • NMDA R2B antibody
    • NMDAR2B antibody
    • NMDE2 antibody
    • NMDE2_HUMAN antibody
    • NME2 antibody
    • NR2B antibody
    • NR3 antibody
    see all

Images

  • Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)
    Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)
    All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/1000 dilution

    Lane 1 : Human hippocampus lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 4 : Mouse liver lysate
    Lane 5 : Mouse kidney lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 7 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
    Lane 8 : Mouse brain lysate
    Lane 9 : Mouse hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 166 kDa
    Observed band size: 166 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,3,4,5,6 and 7: 30 seconds; Lane 8 and 9: 8 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID:9252405; PMID:15470155).

  • Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)
    Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)
    All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/5000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat hippocampus lysate
    Lane 3 : Rat liver lysate
    Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 166 kDa
    Observed band size: 166 kDa


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:9252405; PMID:15470155).

  • Anti-NMDAR2B antibody [EPR19295] (ab183942)
    Anti-NMDAR2B antibody [EPR19295] (ab183942)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (2)

    Publishing research using ab183942? Please let us know so that we can cite the reference in this datasheet.

    ab183942 has been referenced in 2 publications.

    • Gisslen T  et al. Fetal inflammation is associated with persistent systemic and hippocampal inflammation and dysregulation of hippocampal glutamatergic homeostasis. Pediatr Res 85:703-710 (2019). PubMed: 30745569
    • Yang Y  et al. Fuzheng Quxie Decoction Ameliorates Learning and Memory Impairment in SAMP8 Mice by Decreasing Tau Hyperphosphorylation. Evid Based Complement Alternat Med 2017:5934254 (2017). PubMed: 29422936

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab183942.
    Please use the links above to contact us or submit feedback about this product.

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