Recombinant Anti-NMDAR2B antibody [EPR19295] (ab183942)
Overview
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Product name
Anti-NMDAR2B antibody [EPR19295]
See all NMDAR2B primary antibodies -
Description
Rabbit monoclonal [EPR19295] to NMDAR2B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse NMDAR2B aa 1450 to the C-terminus. The exact sequence is proprietary.
Database link: Q01097 -
Positive control
- WB: Human hippocampus lysate; Mouse brain and hippocampus lysates; Rat brain and hippocampus lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19295 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab183942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa). |
Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
Tissue specificity
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
Alternative names
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
Images
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![Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)](https://www.abcam.com/ps/products/183/ab183942/Images/ab183942-258210-anti-nmdar2b-antibody-epr19295-western-blot.jpg)
Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/1000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human fetal liver lysate
Lane 3 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse kidney lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 7 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 8 : Mouse brain lysate
Lane 9 : Mouse hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 166 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3,4,5,6 and 7: 30 seconds; Lane 8 and 9: 8 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID:9252405; PMID:15470155).
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![Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)](https://www.abcam.com/ps/products/183/ab183942/Images/ab183942-258209-anti-nmdar2b-antibody-epr19295-western-blot.jpg)
Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/5000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat hippocampus lysate
Lane 3 : Rat liver lysate
Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 166 kDa
Exposure time: 8 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:9252405; PMID:15470155).
Datasheets and documents
Certificate of Compliance
References
ab183942 has been referenced in 1 publication.
- Yang Y et al. Fuzheng Quxie Decoction Ameliorates Learning and Memory Impairment in SAMP8 Mice by Decreasing Tau Hyperphosphorylation. Evid Based Complement Alternat Med 2017:5934254 (2017). PubMed: 29422936
