Recombinant Anti-NMDAR2B antibody [EPR19295] (ab183942)
![Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)](https://www.abcam.com/ps/products/183/ab183942/Images/ab183942-258210-anti-nmdar2b-antibody-epr19295-western-blot.jpg "Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19295] to NMDAR2B
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NMDAR2B antibody [EPR19295]
See all NMDAR2B primary antibodies -
Description
Rabbit monoclonal [EPR19295] to NMDAR2B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse NMDAR2B aa 1450 to the C-terminus. The exact sequence is proprietary.
Database link: Q01097 -
Positive control
- WB: Human hippocampus lysate; Mouse brain and hippocampus lysates; Rat brain and hippocampus lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19295 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab183942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa). |
Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
Tissue specificity
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
Alternative names
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
Images
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Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/1000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human fetal liver lysate
Lane 3 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse kidney lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 7 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 8 : Mouse brain lysate
Lane 9 : Mouse hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 166 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3,4,5,6 and 7: 30 seconds; Lane 8 and 9: 8 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID:9252405; PMID:15470155).
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![Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)](https://www.abcam.com/ps/products/183/ab183942/Images/ab183942-258209-anti-nmdar2b-antibody-epr19295-western-blot.jpg)
Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/5000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat hippocampus lysate
Lane 3 : Rat liver lysate
Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 166 kDa
Exposure time: 8 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:9252405; PMID:15470155).
-
![Anti-NMDAR2B antibody [EPR19295] (ab183942)](https://www.abcam.com/ps/products/183/ab183942/Images/ab183942-3-benefits-of-recombinant-antibodies.png)
Anti-NMDAR2B antibody [EPR19295] (ab183942)
Datasheets and documents
Certificate of Compliance
References (2)
ab183942 has been referenced in 2 publications.
- Gisslen T et al. Fetal inflammation is associated with persistent systemic and hippocampal inflammation and dysregulation of hippocampal glutamatergic homeostasis. Pediatr Res 85:703-710 (2019). PubMed: 30745569
- Yang Y et al. Fuzheng Quxie Decoction Ameliorates Learning and Memory Impairment in SAMP8 Mice by Decreasing Tau Hyperphosphorylation. Evid Based Complement Alternat Med 2017:5934254 (2017). PubMed: 29422936
