Product nameAnti-NMDAR2B antibody [N59/36]
See all NMDAR2B primary antibodies
DescriptionMouse monoclonal [N59/36] to NMDAR2B
SpecificityNo cross-reactivity with NMDAR2A.
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IP, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Dog
Fusion protein corresponding to Rat NMDAR2B aa 20-271 (N terminal).
AVSGSKARSQKSPPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVE LVAMNETDPKSIITRICDLMSDRKIQGVVFADDTDQEAIAQILDFISAQT LTPILGIHGGSSMIMADKDESSMFFQFGPSIEQQASVMLNIMEEYDWYIF SIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLDDGDSKIQNQ LKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTV PS
Database link: Q00960
- Flow Cyt: SH-SY5Y cells.
The clone number has been updated from S59-36 to N59/36, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab93610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|WB||Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 0.1 - 1 µg/ml. Antigen retrieval is not essential but may optimise staining.|
|IHC-Fr||Use a concentration of 0.1 - 1 µg/ml.|
FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Tissue specificityPrimarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
All lanes : Anti-NMDAR2B antibody [N59/36] (ab93610) at 1/250 dilution
Lane 1 : Induced HEK-T lysates
Lane 2 : Non-Induced HEK-T lysates
Lane 3 : P23X HEK-T negative control lysates
Predicted band size: 166 kDa
Overlay histogram showing SH-SY5Y cells stained with ab93610 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93610, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback
Anti-NMDAR2B antibody [N59/36] (ab93610) at 1/1000 dilution + Rat brain membrane lysate at 15 µg with 1.5% BSA for 30 minutes at RT
Sheep Anti-Mouse IgG: HRP for 1 hour at RT
Predicted band size: 166 kDa
This product has been referenced in:
- Olivero G et al. Prolonged activation of CXCR4 hampers the release-regulating activity of presynaptic NMDA receptors in rat hippocampal synaptosomes. Neurochem Int 126:59-63 (2019). Read more (PubMed: 30858017) »
- Ji Y et al. CRMP2-derived peptide ST2-104 (R9-CBD3) protects SH-SY5Y neuroblastoma cells against Aß25-35-induced neurotoxicity by inhibiting the pCRMP2/NMDAR2B signaling pathway. Chem Biol Interact 305:28-39 (2019). Read more (PubMed: 30871964) »