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    nmdar2b-antibody-nr2b-ab28373.pdf

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Neuroscience Neurotransmission Receptors / Channels Ligand-Gated Ion Channels NMDA Receptors
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Anti-NMDAR2B antibody [NR2B] (ab28373)

  • Datasheet
Reviews (3)Q&A (3)References (17)

Product price, shipping and contact information

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Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (ab28373)
  • Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
  • Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
  • Flow Cytometry - Anti-NMDAR2B antibody [NR2B] (ab28373)

Key features and details

  • Mouse monoclonal [NR2B] to NMDAR2B
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-NMDAR2B antibody [NR2B]
    See all NMDAR2B primary antibodies
  • Description

    Mouse monoclonal [NR2B] to NMDAR2B
  • Host species

    Mouse
  • Specificity

    Detects the N-methyl-D-aspartate (NMDA) receptor type 2B. This antibody has been tested in mouse and rat brain in western blotting. It has not been tested in human for Western blotting - only for ICC on transfected HEK cells.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Rat NMDAR2B aa 934-1457.

  • Positive control

    • WB: Mouse brain tissue ICC: transfected HEK cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein A Chromatography
  • Clonality

    Monoclonal
  • Clone number

    NR2B
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • NMDA Receptors
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human NMDAR2B protein (ab112298)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
WB
Mouse
Application Abreviews Notes
WB (2)
Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa.
IHC-P
1/10 - 1/100.
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Notes
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa.
IHC-P
1/10 - 1/100.
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
  • Tissue specificity

    Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Target information above from: UniProt accession Q13224 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2904 Human
    • Entrez Gene: 14812 Mouse
    • Entrez Gene: 24410 Rat
    • Omim: 138252 Human
    • SwissProt: Q13224 Human
    • SwissProt: Q01097 Mouse
    • SwissProt: Q00960 Rat
    • Unigene: 654430 Human
    • Unigene: 436649 Mouse
    • Unigene: 9711 Rat
    see all
  • Alternative names

    • AW490526 antibody
    • EIEE27 antibody
    • Glutamate [NMDA] receptor subunit epsilon 2 antibody
    • Glutamate [NMDA] receptor subunit epsilon-2 antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
    • Glutamate receptor ionotropic NMDA2B antibody
    • Glutamate receptor subunit epsilon 2 antibody
    • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
    • GRIN 2B antibody
    • GRIN2B antibody
    • hNR 3 antibody
    • hNR3 antibody
    • MGC142178 antibody
    • MGC142180 antibody
    • MRD6 antibody
    • N methyl D asparate receptor channel subunit epsilon 2 antibody
    • N methyl D aspartate receptor subtype 2B antibody
    • N methyl D aspartate receptor subunit 2B antibody
    • N methyl D aspartate receptor subunit 3 antibody
    • N-methyl D-aspartate receptor subtype 2B antibody
    • N-methyl-D-aspartate receptor subunit 3 antibody
    • NMDA NR2B antibody
    • NMDA R2B antibody
    • NMDAR2B antibody
    • NMDE2 antibody
    • NMDE2_HUMAN antibody
    • NME2 antibody
    • NR2B antibody
    • NR3 antibody
    see all

Images

  • Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue

    Predicted band size: 166 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (ab28373)

    ab28373 staining NMDAR2B in Human breast tissue sections (right) compared with negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/20) overnight at 4°C. A HRP-conjugated secondary antibody was used for detection.

  • Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Lanes 2-4 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution
    Lanes 5-7 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilution

    Lanes 3 & 6 : Mouse brain (50ug)
    Lanes 4 & 7 : Rat brain (50ug)

    Predicted band size: 166 kDa

  • Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)This image is courtesy of an anonymous Abreview
    Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution + Rat P14 Cortex Lysate, vesicular fraction at 5 µg

    Secondary
    HRP conjugated Donkey anti-mouse IgG at 1/15000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 166 kDa



    Samples were incubated with primary antibody for 12 hours at 4ºC.

    Blocked with 5% milk for 1 hour at 22ºC. 

    See Abreview

  • Flow Cytometry - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Flow Cytometry - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (17)

    Publishing research using ab28373? Please let us know so that we can cite the reference in this datasheet.

    ab28373 has been referenced in 17 publications.

    • Yang Q  et al. Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus. EBioMedicine 47:470-483 (2019). PubMed: 31474551
    • Zhou D  et al. GLYX-13 Ameliorates Schizophrenia-Like Phenotype Induced by MK-801 in Mice: Role of Hippocampal NR2B and DISC1. Front Mol Neurosci 11:121 (2018). PubMed: 29695955
    • Bacq A  et al. Amygdala GluN2B-NMDAR dysfunction is critical in abnormal aggression of neurodevelopmental origin induced by St8sia2 deficiency. Mol Psychiatry N/A:N/A (2018). PubMed: 30089788
    • Liang X  et al. Tyrosine Phosphorylation of NR2B Contributes to Chronic Migraines via Increased Expression of CGRP in Rats. Biomed Res Int 2017:7203458 (2017). PubMed: 28393079
    • Banerjee J  et al. Altered glutamatergic tone reveals two distinct resting state networks at the cellular level in hippocampal sclerosis. Sci Rep 7:319 (2017). PubMed: 28336943
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-NMDAR2B antibody [NR2B]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
    Sample
    Rat Tissue lysate - other (hippocampi total membrane fraction)
    Specification
    hippocampi total membrane fraction
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 19 2014

    Question

    Dear Abcam manager


    I just send you the mail again to ask about the 2nd antibody for fluoroscence WB detection.

    I sent you the specification information on chemidoc system that we want to use last week but didn't get reply yet.

    According to BioRad web sites, each color emission filter can detect below dyes.



    RED: Cy5, Cy5.5, Alexa Fluor 647 / 680, DyLight 649 / 680, IRDye 680

    GREEN: Cy3, Flamingo, Krypton, Pro-Q Diamond, Alexa Fluor 546, DyLight 549, Rhodamine

    BLUE: Cy2, Coomassie Fluor Orange, Alexa Fluor 488, DyLight 488, Pro-! Emerald, Qdot 605 / 625 / 705

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2012

    Answer

    Thank you for your message and for your patience.
    It is difficult to find three secondary antibodies in three colors that woudl be suitable, but I hope the following will help. Please check the excitation and emission wavelenghts and spectra to ensure they are suitable for the machine you are using. (I say this because some of the blue dyes they suggest go into the green range in the light spectra). I can recommend to review the online datasheets for further information.
    Anti NMDAR1 antibody (ab77264, goat polyclonal)
    Red fluorophore Secondary antibodies for detection of goat primary:
    Donkey anti-Goat IgG H&L (PE) secondary antibody (ab7004)
    Donkey F(ab')2 polyclonal Secondary Antibody to Goat IgG - H&L (PE), pre-adsorbed
    https://www.abcam.com/Donkey-Goat-IgG-HL-PE-preadsorbed-ab7004.html https://www.abcam.com/Donkey-Goat-IgG-HL-PE-preadsorbed-ab7004.html.
    Anti-NMDAR2A antibody (ab14596, rabbit polyclonal)
    Green fluorophore Secondary antibodies for detection of rabbit primary:
    ab6798 Donkey anti-Rabbit IgG H&L (FITC) secondary antibody
    https://www.abcam.com/Donkey-Rabbit-IgG-HL-FITC-ab6798.html https://www.abcam.com/Donkey-Rabbit-IgG-HL-FITC-ab6798.html.

    ab7079 Donkey anti-Rabbit IgG H&L (FITC) secondary antibody
    https://www.abcam.com/Donkey-Rabbit-IgG-HL-FITC-preadsorbed-ab7079.html https://www.abcam.com/Donkey-Rabbit-IgG-HL-FITC-preadsorbed-ab7079.html.
    Anti-NMDAR2B antibody (ab28373, mouse monoclonal)
    The following may fit into the blue channel on the instrument you are using, but I can suggest to please check this. The emission spectra is slightly into the green spectra.
    ab96875 Donkey anti-Mouse IgG H&L (DyLight® 488) secondary antibody
    https://www.abcam.com/Donkey-Mouse-IgG-HL-DyLight-488-ab96875.html https://www.abcam.com/Donkey-Mouse-IgG-HL-DyLight-488-ab96875.html.
    I am sorry we do not have any that are tested and covered by the 6 month guarantee in western blot to suggest on this occasion.
    The other option is to directly conjugate the primary antibodies yourself with our Easylink kits. These are quick and easy to use. However, please note that we recommend the following for these kits:
    The antibody to be conjugated should be in 10-50mM amine-free buffer within a pH range of 6.5-8.5. Modest concentrations of Tris buffer (up to 20mM) are also tolerated.
    I can recommend to read the following important information and frequently asked questions about the kits before selecting a suitable antibody to conjugate:
    https://www.abcam.com/index.html?pageconfig=resource&rid=13148
    https://www.abcam.com/index.html?pageconfig=resource&rid=13156
    I hope this will be helpful. Please do not hesitate to contact me if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Nov 21 2012

    Question

    Currently using ab28373 in hamster tissue (IHC-Fr Fl), but not getting any signal.
    Is there any NR2B antibodies that would work with hamster tissue.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 19 2012

    Answer

    Thank you for calling Abcam earlier today.

    I have continued to try and find the hamster sequence for NR2B to see if there is any antibodies in our catalogue that may work for hamster, but I have not been able to find the hamster sequence.

    There is a chance that ab65783 might work for you, but without the hamster sequence, I would feel unsure recommending it to you. If there is any chance that you could find the sequence and forward that to me, I would gladly do the alignments and see if there is a chance that it would work for you.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on Jul 19 2012

    Question

    Would like to try this antibody with hamster samples.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2012

    Answer

    Thank you for your call today. I've issued the discount code below for testing ab28373in hamster samples.

    DISCOUNT CODE: ***
    Expiration date: July 30, 2012

    This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab28373 in hamster samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Mar 29 2012

    Immunocytochemistry abreview for Anti-NMDAR2B antibody [NR2B]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (Hippocampal neurons)
    Specification
    Hippocampal neurons
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.25% Triton in PBS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 23 2011

    Western blot abreview for Anti-NMDAR2B antibody [NR2B]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Rat P14 Cortex Lysate, vesicular fraction)
    Loading amount
    5 µg
    Specification
    Rat P14 Cortex Lysate, vesicular fraction
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 06 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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