Anti-NMDAR2B antibody [NR2B] (ab28373)
Key features and details
- Mouse monoclonal [NR2B] to NMDAR2B
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-NMDAR2B antibody [NR2B]
See all NMDAR2B primary antibodies -
Description
Mouse monoclonal [NR2B] to NMDAR2B -
Host species
Mouse -
Specificity
Detects the N-methyl-D-aspartate (NMDA) receptor type 2B. This antibody has been tested in mouse and rat brain in western blotting. It has not been tested in human for Western blotting - only for ICC on transfected HEK cells. -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-P HumanWB Mouse -
Immunogen
Recombinant fragment corresponding to Rat NMDAR2B aa 934-1457.
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Positive control
- WB: Mouse brain tissue ICC: transfected HEK cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein A Chromatography -
Clonality
Monoclonal -
Clone number
NR2B -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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IHC-P |
Human
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WB |
Mouse
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Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa.
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IHC-P |
1/10 - 1/100.
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Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa. |
IHC-P
1/10 - 1/100. |
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
Tissue specificity
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
Alternative names
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
Images
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Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue
Predicted band size: 166 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (ab28373)
ab28373 staining NMDAR2B in Human breast tissue sections (right) compared with negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/20) overnight at 4°C. A HRP-conjugated secondary antibody was used for detection.
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Lanes 2-4 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution
Lanes 5-7 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilution
Lanes 3 & 6 : Mouse brain (50ug)
Lanes 4 & 7 : Rat brain (50ug)
Predicted band size: 166 kDa -
Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)This image is courtesy of an anonymous AbreviewAnti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution + Rat P14 Cortex Lysate, vesicular fraction at 5 µg
Secondary
HRP conjugated Donkey anti-mouse IgG at 1/15000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDaSamples were incubated with primary antibody for 12 hours at 4ºC.
Blocked with 5% milk for 1 hour at 22ºC.
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Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
References (17)
ab28373 has been referenced in 17 publications.
- Yang Q et al. Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus. EBioMedicine 47:470-483 (2019). PubMed: 31474551
- Zhou D et al. GLYX-13 Ameliorates Schizophrenia-Like Phenotype Induced by MK-801 in Mice: Role of Hippocampal NR2B and DISC1. Front Mol Neurosci 11:121 (2018). PubMed: 29695955
- Bacq A et al. Amygdala GluN2B-NMDAR dysfunction is critical in abnormal aggression of neurodevelopmental origin induced by St8sia2 deficiency. Mol Psychiatry N/A:N/A (2018). PubMed: 30089788
- Liang X et al. Tyrosine Phosphorylation of NR2B Contributes to Chronic Migraines via Increased Expression of CGRP in Rats. Biomed Res Int 2017:7203458 (2017). PubMed: 28393079
- Banerjee J et al. Altered glutamatergic tone reveals two distinct resting state networks at the cellular level in hippocampal sclerosis. Sci Rep 7:319 (2017). PubMed: 28336943