Anti-NMDAR2B antibody [NR2B] (ab28373)
![Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)](https://www.abcam.com/ps/products/28/ab28373/Images/ab28373-39291-anti-nmdar2b-antibody-nr2b-western-blot.png "Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)"){kind=small}
Key features and details
- Mouse monoclonal [NR2B] to NMDAR2B
- Suitable for: WB, IHC-P, ICC, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-NMDAR2B antibody [NR2B]
See all NMDAR2B primary antibodies -
Description
Mouse monoclonal [NR2B] to NMDAR2B -
Host species
Mouse -
Specificity
Detects the N-methyl-D-aspartate (NMDA) receptor type 2B. This antibody has been tested in mouse and rat brain in western blotting. It has not been tested in human for Western blotting - only for ICC on transfected HEK cells. -
Tested applications
Suitable for: WB, IHC-P, ICC, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Rat NMDAR2B aa 934-1457.
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Positive control
- WB: Mouse brain tissue ICC: transfected HEK cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A Chromatography -
Clonality
Monoclonal -
Clone number
NR2B -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa.
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| IHC-P |
1/10 - 1/100.
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| ICC | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa. |
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IHC-P
1/10 - 1/100. |
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ICC
Use at an assay dependent concentration. |
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Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
Tissue specificity
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
Alternative names
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
Images
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Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue
Predicted band size: 166 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (ab28373)](https://www.abcam.com/ps/products/28/ab28373/Images/ab28373-306783-anti-nmdar2b-antibody-nr2b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (ab28373)ab28373 staining NMDAR2B in Human breast tissue sections (right) compared with negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/20) overnight at 4°C. A HRP-conjugated secondary antibody was used for detection.
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![Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)](https://www.abcam.com/ps/products/28/ab28373/Images/ab28373-39290-anti-nmdar2b-antibody-nr2b-western-blot.jpg)
Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)Lanes 2-4 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution
Lanes 5-7 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilution
Lanes 3 & 6 : Mouse brain (50ug)
Lanes 4 & 7 : Rat brain (50ug)
Predicted band size: 166 kDa -
![Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)](https://www.abcam.com/ps/products/28/ab28373/Images/ab28373-306591-anti-nmdar2b-antibody-nr2b-western-blot.jpg)
Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)This image is courtesy of an anonymous AbreviewAnti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution + Rat P14 Cortex Lysate, vesicular fraction at 5 µg
Secondary
HRP conjugated Donkey anti-mouse IgG at 1/15000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDaSamples were incubated with primary antibody for 12 hours at 4ºC.
Blocked with 5% milk for 1 hour at 22ºC.
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![Flow Cytometry - Anti-NMDAR2B antibody [NR2B] (ab28373)](https://www.abcam.com/ps/products/28/ab28373/Images/ab28373-39289-anti-nmdar2b-antibody-nr2b-flow-cytometry.jpg)
Flow Cytometry - Anti-NMDAR2B antibody [NR2B] (ab28373)Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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Datasheet download
References (28)
ab28373 has been referenced in 28 publications.
- Loera-Valencia R et al. High levels of 27-hydroxycholesterol results in synaptic plasticity alterations in the hippocampus. Sci Rep 11:3736 (2021). PubMed: 33580102
- Imbriglio T et al. Developmental up-regulation of NMDA receptors in the prefrontal cortex and hippocampus of mGlu5 receptor knock-out mice. Mol Brain 14:77 (2021). PubMed: 33962661
- Verhaeghe R et al. Maternal stress programs a demasculinization of glutamatergic transmission in stress-related brain regions of aged rats. Geroscience N/A:N/A (2021). PubMed: 33983623
- Esteras N et al. Mitochondrial ROS control neuronal excitability and cell fate in frontotemporal dementia. Alzheimers Dement N/A:N/A (2021). PubMed: 34057756
- Gu SX et al. Memantine Protects From Exacerbation of Ischemic Stroke and Blood Brain Barrier Disruption in Mild But Not Severe Hyperhomocysteinemia. J Am Heart Assoc 9:e013368 (2020). PubMed: 32067580
