• Product name
    Anti-NMDAR2B antibody [NR2B]
    See all NMDAR2B primary antibodies
  • Description
    Mouse monoclonal [NR2B] to NMDAR2B
  • Host species
  • Specificity
    Detects the N-methyl-D-aspartate (NMDA) receptor type 2B. This antibody has been tested in mouse and rat brain in western blotting. It has not been tested in human for Western blotting - only for ICC on transfected HEK cells.
  • Tested applications
    Suitable for: ICC, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Rat NMDAR2B aa 934-1457.

  • Positive control
    • WB: Mouse brain tissue ICC: transfected HEK cells



Our Abpromise guarantee covers the use of ab28373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 1 µg/ml.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa.
IHC-P 1/10 - 1/100.
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
  • Tissue specificity
    Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW490526 antibody
    • EIEE27 antibody
    • Glutamate [NMDA] receptor subunit epsilon 2 antibody
    • Glutamate [NMDA] receptor subunit epsilon-2 antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
    • Glutamate receptor ionotropic NMDA2B antibody
    • Glutamate receptor subunit epsilon 2 antibody
    • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
    • GRIN 2B antibody
    • GRIN2B antibody
    • hNR 3 antibody
    • hNR3 antibody
    • MGC142178 antibody
    • MGC142180 antibody
    • MRD6 antibody
    • N methyl D asparate receptor channel subunit epsilon 2 antibody
    • N methyl D aspartate receptor subtype 2B antibody
    • N methyl D aspartate receptor subunit 2B antibody
    • N methyl D aspartate receptor subunit 3 antibody
    • N-methyl D-aspartate receptor subtype 2B antibody
    • N-methyl-D-aspartate receptor subunit 3 antibody
    • NMDA NR2B antibody
    • NMDA R2B antibody
    • NMDAR2B antibody
    • NMDE2 antibody
    • NMDE2_HUMAN antibody
    • NME2 antibody
    • NR2B antibody
    • NR3 antibody
    see all


  • Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue

    Predicted band size: 166 kDa

  • ab28373 staining NMDAR2B in Human breast tissue sections (right) compared with negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/20) overnight at 4°C. A HRP-conjugated secondary antibody was used for detection.

  • Lanes 2-4 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution
    Lanes 5-7 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilution

    Lanes 3 & 6 : Mouse brain (50ug)
    Lanes 4 & 7 : Rat brain (50ug)

    Predicted band size: 166 kDa

  • Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution + Rat P14 Cortex Lysate, vesicular fraction at 5 µg

    HRP conjugated Donkey anti-mouse IgG at 1/15000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 166 kDa

    Samples were incubated with primary antibody for 12 hours at 4ºC.

    Blocked with 5% milk for 1 hour at 22ºC. 

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


This product has been referenced in:
  • Zhou D  et al. GLYX-13 Ameliorates Schizophrenia-Like Phenotype Induced by MK-801 in Mice: Role of Hippocampal NR2B and DISC1. Front Mol Neurosci 11:121 (2018). Read more (PubMed: 29695955) »
  • Banerjee J  et al. Altered glutamatergic tone reveals two distinct resting state networks at the cellular level in hippocampal sclerosis. Sci Rep 7:319 (2017). Read more (PubMed: 28336943) »
See all 9 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A


Thank you for your message and for your patience.
It is difficult to find three secondary antibodies in three colors that woudl be suitable, but I hope the following will help. Please check the excitation and emission wavelenghts and spectra to ensure they are suitable for the machine you are using. (I say this because some of the blue dyes they suggest go into the green range in the light spectra). I can recommend to review the online datasheets for further information.
Anti NMDAR1 antibody (ab77264, goat polyclonal)
Red fluorophore Secondary antibodies for detection of goat primary:
Donkey anti-Goat IgG H&L (PE) secondary antibody (ab7004)
Donkey F(ab')2 polyclonal Secondary Antibody to Goat IgG - H&L (PE), pre-adsorbed
https://www.abcam.com/index.html?datasheet=7004 https://www.abcam.com/index.html?datasheet=7004.
Anti-NMDAR2A antibody (ab14596, rabbit polyclonal)
Green fluorophore Secondary antibodies for detection of rabbit primary:
ab6798 Donkey anti-Rabbit IgG H&L (FITC) secondary antibody
https://www.abcam.com/index.html?datasheet=6798 https://www.abcam.com/index.html?datasheet=6798.

ab7079 Donkey anti-Rabbit IgG H&L (FITC) secondary antibody
https://www.abcam.com/index.html?datasheet=7079 https://www.abcam.com/index.html?datasheet=7079.
Anti-NMDAR2B antibody (ab28373, mouse monoclonal)
The following may fit into the blue channel on the instrument you are using, but I can suggest to please check this. The emission spectra is slightly into the green spectra.
ab96875 Donkey anti-Mouse IgG H&L (DyLight® 488) secondary antibody
https://www.abcam.com/index.html?datasheet=96875 https://www.abcam.com/index.html?datasheet=96875.
I am sorry we do not have any that are tested and covered by the 6 month guarantee in western blot to suggest on this occasion.
The other option is to directly conjugate the primary antibodies yourself with our Easylink kits. These are quick and easy to use. However, please note that we recommend the following for these kits:
The antibody to be conjugated should be in 10-50mM amine-free buffer within a pH range of 6.5-8.5. Modest concentrations of Tris buffer (up to 20mM) are also tolerated.
I can recommend to read the following important information and frequently asked questions about the kits before selecting a suitable antibody to conjugate:
I hope this will be helpful. Please do not hesitate to contact me if you have any further questions.

Read More


Thank you for calling Abcam earlier today.

I have continued to try and find the hamster sequence for NR2B to see if there is any antibodies in our catalogue that may work for hamster, but I have not been able to find the hamster sequence.

There is a chance that ab65783 might work for you, but without the hamster sequence, I would feel unsure recommending it to you. If there is any chance that you could find the sequence and forward that to me, I would gladly do the alignments and see if there is a chance that it would work for you.

Please let me know if there is anything else I can help you with.

Read More


Thank you for your call today. I've issued the discount code below for testing ab28373in hamster samples.

Expiration date: July 30, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab28373 in hamster samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up