Western blot abreview for Anti-NMDAR2B antibody [NR2B]

Good
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat P14 Cortex Lysate, vesicular fraction)
Loading amount
5 µg
Specification
Rat P14 Cortex Lysate, vesicular fraction
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS-Tween

Secondary antibody

Name
Non-Abcam antibody was used: Donkey Anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/15000

Detection

Detection method
ECl (Immobilon, Millipore)
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: ~170 kDa
Positive control
The vesicular fraction of cortical lysate should contain NMDA receptors.

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Submitted Dec 06 2011

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