Immunocytochemistry abreview for Anti-NMDAR2B antibody [NR2B]

Average
Abreviews
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Hippocampal neurons)
Specification
Hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/50
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 1% BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: donkey Anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

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Submitted Dec 23 2011

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