Western blot abreview for Anti-NMDAR2B antibody [NR2B]

Poor
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
Sample
Rat Tissue lysate - other (hippocampi total membrane fraction)
Specification
hippocampi total membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
19 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% blocking milk in 0.1% TBST
Dilution
1/5000

Secondary antibody

Name

Detection

Detection method
ECL
Bands
Specific: 610 kDa
Exposure
20 second(s)

Additional data

Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise. We suggest reducing and denaturing the samples and running under reduced, denatured conditions.

Abcam user community

Verified customer

Submitted Feb 19 2014

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