Recombinant Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)
![Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.com/ps/products/81/ab81271/Images/ab81271-296742-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg "Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303)
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NMDAR2B (phospho S1303) antibody [EP1858Y]
See all NMDAR2B primary antibodies -
Description
Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303) -
Host species
Rabbit -
Specificity
ab81271 detects NMDAR2B when phosphorylated at serine 1303 -
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A phospho specific peptide corresponding to residues surrounding serine 1303 of human NMDAR2B.
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Positive control
- WB: Human, mouse and rat brain tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1858Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab81271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | 1/1000. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 166 kDa. |
Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
Tissue specificity
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
Alternative names
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
Images
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Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat brain tissue lysate. The membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.com/ps/products/81/ab81271/Images/ab81271-296741-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg)
Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human brain tissue lysate. The membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
-
![Dot Blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.com/ps/products/81/ab81271/Images/ab81271-296735-anti-nmdar2b-phospho-s1303-antibody-ep1858y-dot-blot.jpg)
Dot Blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)Dot blot analysis of NMDAR2B (phospho S1303) phospho peptide (Lane 1) and NMDAR2B non-phospho peptide (Lane 2) labelling NMDAR2B (phospho S1303) with ab81271 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.com/ps/products/81/ab81271/Images/ab81271-96453-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg)
Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Mouse brain lysates (untreated)
Lane 2 : Mouse brain lysates (treated with AP)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labelled IgG at 1/2000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted? -
![Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.com/ps/products/81/ab81271/Images/ab81271-5-benefits-of-recombinant-antibodies.png)
Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)
Protocols
References (20)
ab81271 has been referenced in 20 publications.
- Li N et al. LPM580098, a Novel Triple Reuptake Inhibitor of Serotonin, Noradrenaline, and Dopamine, Attenuates Neuropathic Pain. Front Pharmacol 10:53 (2019). PubMed: 30837867
- Wang XS et al. Anxiolytic effects of Formononetin in an inflammatory pain mouse model. Mol Brain 12:36 (2019). PubMed: 30961625
- Castany S et al. Repeated Sigma-1 Receptor Antagonist MR309 Administration Modulates Central Neuropathic Pain Development After Spinal Cord Injury in Mice. Front Pharmacol 10:222 (2019). PubMed: 30967775
- Kumar M et al. Alteration in the phosphorylation status of NMDA receptor GluN2B subunit by activation of both NMDA receptor and L-type voltage gated calcium channel. Neurosci Lett 709:134343 (2019). PubMed: 31279915
- Ji Z et al. Kibra Modulates Learning and Memory via Binding to Dendrin. Cell Rep 26:2064-2077.e7 (2019). PubMed: 30784589
