Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Gel Running Conditions
Non-reduced Denaturing (15% SDS-PAGE)
Loading amount
8 µg
Treatment
RIPA & 1%Protease inhibitor for 15min on ice
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: ZSJQ IgG/HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 166 kDa
Additional data
Additional Notes
After many times experiments, we found the antibody was very stable, and there were two light bands for the protein when the loading quantities was enough. The left side were fresh samples, and the right side were samples that keeped in -20? for 1 year (even more). Degradation of samples might result in poor results.
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Submitted Nov 08 2018