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Rabbit polyclonal to NMDAR2B (phospho S1480)
ab73014 is specific for ~180k NMDAR2B protein phosphorylated at Ser1480. Immunolabeling of the NMDAR2B band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is also blocked by lambda-phosphatase treatment.
Predicted to work with:
Mouse, Chicken, Cow, Dog, Human, Non human primates, Zebrafish
Synthetic phosphopeptide corresponding to amino acid residues surrounding phospho-Ser1480 of rat NMDAR2B.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES
Concentration information loading...
Immunogen affinity purified
ab73014 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
Information by UniProt
Glutamate [NMDA] receptor subunit epsilon 2 antibody
Western blot - Anti-NMDAR2B (phospho S1480) antibody (ab73014)
All lanes :
Anti-NMDAR2B (phospho S1480) antibody (ab73014) at 1/1000 dilution
Lane 1 :
Rat hippocampal lysate
Lane 2 :
Rat hippocampal lysate incubated in lambda-phosphatase (1200 units for 30 min)
Predicted band size:
Observed band size:
180 kDa (
why is the actual band size different from the predicted?
Additional bands at:
85 kDa. We are unsure as to the identity of these extra bands.
The phosphospecificity of the labeling is shown in the second lane (lambda-phosphatase). The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
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