N methyl D asparate receptor channel subunit epsilon 2 antibody
N methyl D aspartate receptor subtype 2B antibody
N methyl D aspartate receptor subunit 2B antibody
N methyl D aspartate receptor subunit 3 antibody
N-methyl D-aspartate receptor subtype 2B antibody
N-methyl-D-aspartate receptor subunit 3 antibody
NMDA NR2B antibody
NMDA R2B antibody
Western blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Lanes 1-3 : Anti-NMDAR2B (phospho Y1252) antibody (ab18532) at 3 µg/ml Lane 4 : NR2B antibody (positive control)
Lane 1 : mouse cortical extract with Mouse NMDAR2B (unmodified ) peptide (ab19637) at 2 µg/ml Lane 2 : mouse cortical extract with Mouse NMDAR2B (phospho Y1252) peptide (ab19636) at 2 µg/ml Lanes 3-4 : mouse cortical extract
Lysates/proteins at 20 µg per lane.
Predicted band size: 166 kDa Observed band size: 166 kDa Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NMDAR2B (phospho Y1252) antibody (ab18532)This image is courtesy of an abreview submitted by Sophie Pezet, Laboratoire de Neurobiologie, France
IHC-FoFr image of NMDAR2B(pY1252) staining on mouse brain sections (A) Hypothalamic paraventricular nucleus and (B) Cerebral cortex using ab18532(1:100). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Delint-Ramirez I et al. In vivo composition of NMDA receptor signaling complexes differs between membrane subdomains and is modulated by PSD-95 and PSD-93. J Neurosci30:8162-70 (2010).
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