Anti-NME2 antibody [4G7A8] (ab204958)
![Western blot - Anti-NME2 antibody [4G7A8] (ab204958)](https://www.abcam.com/ps/products/204/ab204958/Images/ab204958-247615-anti-nme2-antibody-4g7a8-western-blot.jpg "Western blot - Anti-NME2 antibody [4G7A8] (ab204958)")
Key features and details
- Mouse monoclonal [4G7A8] to NME2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NME2 antibody [4G7A8]
See all NME2 primary antibodies -
Description
Mouse monoclonal [4G7A8] to NME2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Orangutan
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Immunogen
Recombinant full length protein corresponding to Human NME2 aa 1 to the C-terminus. Expressed in E. coli.
Database link: P22392-1 -
Positive control
- Human full length recombinant NME2 (amino acids 1-152). Lysate from HEK293 cells transfected with full length recombinant NME2 (amino acids 1-152). Human ovarian cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4G7A8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 17 kDa.
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| IHC-P |
1/200 - 1/1000.
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| Notes |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 17 kDa. |
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IHC-P
1/200 - 1/1000. |
Target
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Function
Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the NDK family. -
Cellular localization
Cytoplasm. Nucleus. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 395916 Chicken
- Entrez Gene: 615447 Cow
- Entrez Gene: 480559 Dog
- Entrez Gene: 4831 Human
- Entrez Gene: 654364 Human
- Entrez Gene: 18103 Mouse
- Entrez Gene: 100174205 Orangutan
- Entrez Gene: 733683 Pig
see all -
Alternative names
- C myc purine binding transcription factor PUF antibody
- C myc transcription factor antibody
- C-myc purine-binding transcription factor PUF antibody
see all
Images
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Western blot - Anti-NME2 antibody [4G7A8] (ab204958)Anti-NME2 antibody [4G7A8] (ab204958) at 1/500 dilution + Human full length recombinant NME2 (amino acids 1-152).
Predicted band size: 17 kDa(Expected MW is 43.2 kDa)
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![Western blot - Anti-NME2 antibody [4G7A8] (ab204958)](https://www.abcam.com/ps/products/204/ab204958/Images/ab204958-247614-anti-nme2-antibody-4g7a8-western-blot.jpg)
Western blot - Anti-NME2 antibody [4G7A8] (ab204958)All lanes : Anti-NME2 antibody [4G7A8] (ab204958) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Lysate from HEK293 cells transfected with full length recombinant NME2 (amino acids 1-152).
Predicted band size: 17 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [4G7A8] (ab204958)](https://www.abcam.com/ps/products/204/ab204958/Images/ab204958-247616-anti-nme2-antibody-4g7a8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [4G7A8] (ab204958)Immunohistochemical analysis of paraffin-embedded, DAB-stained human ovarian cancer tissue, labeling NME2 using ab204958 at a 1/200 dilution
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab204958 has not yet been referenced specifically in any publications.
