Anti-NME2 antibody [4G7A8] (ab204958)

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Key features and details

  • Mouse monoclonal [4G7A8] to NME2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-NME2 antibody [4G7A8]
    See all NME2 primary antibodies

  • Description

    Mouse monoclonal [4G7A8] to NME2

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Orangutan

  • Immunogen

    Recombinant full length protein corresponding to Human NME2 aa 1-152. Expressed in E. coli.
    Sequence:

    MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQ HYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSK PGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWV YE


    Database link: P22392-1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human full length recombinant NME2 (amino acids 1-152). Lysate from HEK293 cells transfected with full length recombinant NME2 (amino acids 1-152). Human ovarian cancer tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab204958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 17 kDa.
IHC-P 1/200 - 1/1000.

Target

Images

  • Western blot - Anti-NME2 antibody [4G7A8] (ab204958)
    Western blot - Anti-NME2 antibody [4G7A8] (ab204958)
    Anti-NME2 antibody [4G7A8] (ab204958) at 1/500 dilution + Human full length recombinant NME2 (amino acids 1-152).

    Predicted band size: 17 kDa



    (Expected MW is 43.2 kDa)

  • Western blot - Anti-NME2 antibody [4G7A8] (ab204958)
    Western blot - Anti-NME2 antibody [4G7A8] (ab204958)
    All lanes : Anti-NME2 antibody [4G7A8] (ab204958) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Lysate from HEK293 cells transfected with full length recombinant NME2 (amino acids 1-152).

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [4G7A8] (ab204958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [4G7A8] (ab204958)

    Immunohistochemical analysis of paraffin-embedded, DAB-stained human ovarian cancer tissue, labeling NME2 using ab204958 at a 1/200 dilution

References (0)

Publishing research using ab204958? Please let us know so that we can cite the reference in this datasheet.

ab204958 has not yet been referenced specifically in any publications.

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