Key features and details
- Mouse monoclonal [4G7A8] to NME2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-NME2 antibody [4G7A8]
See all NME2 primary antibodies
DescriptionMouse monoclonal [4G7A8] to NME2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Orangutan
Recombinant full length protein corresponding to Human NME2 aa 1-152. Expressed in E. coli.
MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQ HYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSK PGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWV YE
Database link: P22392-1
- Human full length recombinant NME2 (amino acids 1-152). Lysate from HEK293 cells transfected with full length recombinant NME2 (amino acids 1-152). Human ovarian cancer tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab204958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 17 kDa.|
|IHC-P||1/200 - 1/1000.|
FunctionMajor role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the NDK family.
Cellular localizationCytoplasm. Nucleus. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus.
- Information by UniProt
- C myc purine binding transcription factor PUF antibody
- C myc transcription factor antibody
- C-myc purine-binding transcription factor PUF antibody
Anti-NME2 antibody [4G7A8] (ab204958) at 1/500 dilution + Human full length recombinant NME2 (amino acids 1-152).
Predicted band size: 17 kDa
(Expected MW is 43.2 kDa)
All lanes : Anti-NME2 antibody [4G7A8] (ab204958) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Lysate from HEK293 cells transfected with full length recombinant NME2 (amino acids 1-152).
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin-embedded, DAB-stained human ovarian cancer tissue, labeling NME2 using ab204958 at a 1/200 dilution
ab204958 has not yet been referenced specifically in any publications.