Anti-NME2 antibody (ab154274)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-NME2 antibody
See all NME2 primary antibodies -
Description
Rabbit polyclonal to NME2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Monkey, Xenopus tropicalis
-
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-105 of Human NME2 (P22392)
-
Positive control
- Mouse brain and A549 whole cell lysate; HeLa and HCT116 cell lines.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab154274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 17 kDa. |
Target
-
Function
Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the NDK family. -
Cellular localization
Cytoplasm. Nucleus. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 395916 Chicken
- Entrez Gene: 615447 Cow
- Entrez Gene: 480559 Dog
- Entrez Gene: 4831 Human
- Entrez Gene: 654364 Human
- Entrez Gene: 18103 Mouse
- Entrez Gene: 733683 Pig
- Entrez Gene: 100533972 Rabbit
see all -
Alternative names
- C myc purine binding transcription factor PUF antibody
- C myc transcription factor antibody
- C-myc purine-binding transcription factor PUF antibody
see all
Images
-
Western blot - Anti-NME2 antibody (ab154274)Anti-NME2 antibody (ab154274) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 17 kDa
12% SDS PAGE -
Western blot - Anti-NME2 antibody (ab154274)Anti-NME2 antibody (ab154274) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 17 kDa
12% SDS PAGE
Protocols
References
This product has been referenced in:
- Moreira DS & Murta SM Involvement of nucleoside diphosphate kinase b and elongation factor 2 in Leishmania braziliensis antimony resistance phenotype. Parasit Vectors 9:641 (2016). Read more (PubMed: 27964761) »
