Anti-NME2 antibody (ab154274)
Key features and details
- Rabbit polyclonal to NME2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NME2 antibody
See all NME2 primary antibodies -
Description
Rabbit polyclonal to NME2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Monkey, Xenopus tropicalis -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-105 of Human NME2 (P22392)
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Positive control
- Mouse brain and A549 whole cell lysate; HeLa and HCT116 cell lines.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/3000. Predicted molecular weight: 17 kDa. |
Target
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Function
Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the NDK family. -
Cellular localization
Cytoplasm. Nucleus. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 395916 Chicken
- Entrez Gene: 615447 Cow
- Entrez Gene: 480559 Dog
- Entrez Gene: 4831 Human
- Entrez Gene: 654364 Human
- Entrez Gene: 18103 Mouse
- Entrez Gene: 733683 Pig
- Entrez Gene: 100533972 Rabbit
see all -
Alternative names
- C myc purine binding transcription factor PUF antibody
- C myc transcription factor antibody
- C-myc purine-binding transcription factor PUF antibody
see all
Images
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Anti-NME2 antibody (ab154274) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 17 kDa
12% SDS PAGE -
Anti-NME2 antibody (ab154274) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 17 kDa
12% SDS PAGE
References (1)
ab154274 has been referenced in 1 publication.
- Moreira DS & Murta SM Involvement of nucleoside diphosphate kinase b and elongation factor 2 in Leishmania braziliensis antimony resistance phenotype. Parasit Vectors 9:641 (2016). PubMed: 27964761