Overview

  • Product name

    Anti-NME2 antibody [EPR8351]
    See all NME2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8351] to NME2
  • Host species

    Rabbit
  • Specificity

    The immunogen used to raise this antibody shares 85% homology with human NME1. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NME2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • LnCaP, PC-12, CHO, 293T, Human spleen and Human thyroid lysates; Human kidney tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the NDK family.
    • Cellular localization

      Cytoplasm. Nucleus. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus.
    • Information by UniProt
    • Database links

    • Alternative names

      • C myc purine binding transcription factor PUF antibody
      • C myc transcription factor antibody
      • C-myc purine-binding transcription factor PUF antibody
      • epididymis secretory sperm binding protein Li 155an antibody
      • HEL-S-155an antibody
      • Histidine protein kinase NDKB antibody
      • MGC111212 antibody
      • MGC2212 antibody
      • NDK B antibody
      • NDKB antibody
      • NDKB_HUMAN antibody
      • NDP kinase B antibody
      • NDPK B antibody
      • NDPKB antibody
      • NM23 H2 antibody
      • nm23-H2 antibody
      • NM23B antibody
      • NME/NM23 nucleoside diphosphate kinase 2 antibody
      • nme2 antibody
      • Non metastatic cells 2, protein (NM23B) expressed in antibody
      • non-metastatic cells 2, protein (NM23) expressed in antibody
      • Nucleoside diphosphate kinase B antibody
      • Nucleotide diphosphate kinase B antibody
      • PUF antibody
      see all

    Images

    • All lanes : Anti-NME2 antibody [EPR8351] (ab131329) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : PC-12 cell lysate
      Lane 3 : CHO cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : Human spleen lysate
      Lane 6 : Human thyroid lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling NME2 with ab131329 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Qiu J  et al. Cellular nucleic acid binding protein suppresses tumor cell metastasis and induces tumor cell death by downregulating heterogeneous ribonucleoprotein K in fibrosarcoma cells. Biochim Biophys Acta 1840:2244-52 (2014). Human . Read more (PubMed: 24594223) »
    • Chen S  et al. Mechanistic studies for the role of cellular nucleic-acid-binding protein (CNBP) in regulation of c-myc transcription. Biochim Biophys Acta 1830:4769-77 (2013). WB, IP ; Human . Read more (PubMed: 23774591) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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