Recombinant
RabMAb

Recombinant Anti-NME2 antibody [EPR8351] (Alexa Fluor® 594) (ab211597)

Overview

  • Product name

    Anti-NME2 antibody [EPR8351] (Alexa Fluor® 594)
    See all NME2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8351] to NME2 (Alexa Fluor® 594)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NME2 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P22392

  • Positive control

    • Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/500.

Target

  • Function

    Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the NDK family.
  • Cellular localization

    Cytoplasm. Nucleus. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C myc purine binding transcription factor PUF antibody
    • C myc transcription factor antibody
    • C-myc purine-binding transcription factor PUF antibody
    • epididymis secretory sperm binding protein Li 155an antibody
    • HEL-S-155an antibody
    • Histidine protein kinase NDKB antibody
    • MGC111212 antibody
    • MGC2212 antibody
    • NDK B antibody
    • NDKB antibody
    • NDKB_HUMAN antibody
    • NDP kinase B antibody
    • NDPK B antibody
    • NDPKB antibody
    • NM23 H2 antibody
    • nm23-H2 antibody
    • NM23B antibody
    • NME/NM23 nucleoside diphosphate kinase 2 antibody
    • nme2 antibody
    • Non metastatic cells 2, protein (NM23B) expressed in antibody
    • non-metastatic cells 2, protein (NM23) expressed in antibody
    • Nucleoside diphosphate kinase B antibody
    • Nucleotide diphosphate kinase B antibody
    • PUF antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab211597 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211597, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab211597 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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