Overview

  • Product name

    Anti-NME3 antibody [EPR13117]
    See all NME3 primary antibodies
  • Description

    Rabbit monoclonal [EPR13117] to NME3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NME3 aa 50-150. The exact sequence is proprietary.
    Database link: Q13232

  • Positive control

    • MOLT4, Raji and HeLa cell lysates; Human cardiac muscle and kidney tissues; HeLa and MOLT4 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/50 - 1/70.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Probably has a role in normal hematopoiesis by inhibition of granulocyte differentiation and induction of apoptosis.
    • Sequence similarities

      Belongs to the NDK family.
    • Developmental stage

      Preferentially expressed at early stages of myeloid differentiation of highly purified CD34+ cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • c371H6.2 antibody
      • DR-nm23 antibody
      • Expressed in non metastatic cells 3 antibody
      • NDK 3 antibody
      • NDK3 antibody
      • NDK3_HUMAN antibody
      • NDP kinase 3 antibody
      • NDP kinase C antibody
      • NDPK C antibody
      • NDPKC antibody
      • NM23 H3 antibody
      • nm23-H3 antibody
      • NM23H3 antibody
      • NME 3 antibody
      • NME/NM23 nucleoside diphosphate kinase 3 antibody
      • Nme3 antibody
      • Non metastatic cells 3 protein expressed in antibody
      • Nucleoside diphosphate kinase 3 antibody
      • Nucleoside diphosphate kinase C antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot analysis of immunoprecipitation pellet from MOLT4 cell lysate immunoprecipitated using ab181257 at 1/50 dilution.
      Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-NME3 antibody [EPR13117] (ab181257) at 1/2000 dilution

      Lane 1 : MOLT4 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 19 kDa

    References

    ab181257 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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