Product nameAnti-Nmnat1 antibody
See all Nmnat1 primary antibodies
DescriptionRabbit polyclonal to Nmnat1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Nmnat1. Note: the amino acid sequence is proprietary)(Peptide available as ab45547.)
- ab45548 gave a positive result in the following human tissue lysates: Brain, Testis, Liver, Heart, Skeletal Muscle, Kidney, Colon (data not shown).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab45548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 29 kDa (predicted molecular weight: 32 kDa).|
FunctionCatalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed:17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (PubMed:17402747). Can use triazofurin monophosphate (TrMP) as substrate (PubMed:17402747). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (PubMed:17402747). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (PubMed:17402747). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (PubMed:17402747). Protects against axonal degeneration following mechanical or toxic insults.
Tissue specificityWidely expressed with highest levels in skeletal muscle, heart and kidney. Also expressed in the liver pancreas and placenta. Widely expressed throughout the brain.
PathwayCofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.
Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.
Involvement in diseaseLeber congenital amaurosis 9
Sequence similaritiesBelongs to the eukaryotic NMN adenylyltransferase family.
- Information by UniProt
- EC 188.8.131.52 antibody
- LCA9 antibody
- Leber's congenital amaurosis 9 antibody
All lanes : Anti-Nmnat1 antibody (ab45548) at 1/250 dilution
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 6 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Dall M et al. Hepatic NAD+ levels and NAMPT abundance are unaffected during prolonged high-fat diet consumption in C57BL/6JBomTac mice. Mol Cell Endocrinol 473:245-256 (2018). WB . Read more (PubMed: 29408602) »
- Giammona LM et al. Mechanistic studies on the effects of nicotinamide on megakaryocytic polyploidization and the roles of NAD+ levels and SIRT inhibition. Exp Hematol 37:1340-1352.e3 (2009). WB ; Human . Read more (PubMed: 19715739) »