Overview

  • Product name

  • Description

    Rabbit polyclonal to NMNAT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Rat NMNAT2 aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab138807)

  • Positive control

    • WB: This antibody gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Rat Cortex; Mouse Cortex; Rat Forebrain. ICC/IF: This antibody gave a positive result in IF in the following Methanol fixed cell line: SKNSH

Properties

Applications

Our Abpromise guarantee covers the use of ab110040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannnot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+).
  • Tissue specificity

    Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans.
  • Pathway

    Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.
  • Sequence similarities

    Belongs to the eukaryotic NMN adenylyltransferase family.
  • Cellular localization

    Cytoplasm. Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1orf15 antibody
    • KIAA0479 antibody
    • MGC2756 antibody
    • NaMN adenylyltransferase 2 antibody
    • Nicotinamide mononucleotide adenylyltransferase 2 antibody
    • Nicotinamide nucleotide adenylyltransferase 2 antibody
    • Nicotinate-nucleotide adenylyltransferase 2 antibody
    • NMN adenylyltransferase 2 antibody
    • NMNA2_HUMAN antibody
    • NMNAT 2 antibody
    • Nmnat2 antibody
    • PNAT 2 antibody
    • PNAT2 antibody
    • Pyridine nucleotide adenylyltransferase 2 antibody
    see all

Images

  • All lanes : Anti-NMNAT2 antibody (ab110040) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Rat Cortex Tissue Lysate
    Lane 4 : Mouse Cortex Tissue Lysate
    Lane 5 : Rat Forebrain Rat Tissue Lysate
    Lane 6 : Lung (Rat) Tissue Lysate
    Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 24 kDa, 68 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute


    Both Rat Lung tissue lysate and NIH3T3 whole cell lysate were used as negative controls for Nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2)
  • ab110040 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110040 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Ali YO  et al. Screening with an NMNAT2-MSD platform identifies small molecules that modulate NMNAT2 levels in cortical neurons. Sci Rep 7:43846 (2017). Read more (PubMed: 28266613) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain from newborn pup)
Loading amount
40 µg
Specification
brain from newborn pup
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Jon Gilley

Verified customer

Submitted Jan 10 2013

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