Product nameAnti-NMNAT2 antibody
See all NMNAT2 primary antibodies
DescriptionRabbit polyclonal to NMNAT2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chinese hamster
Synthetic peptide corresponding to Rat NMNAT2 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- WB: This antibody gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Rat Cortex; Mouse Cortex; Rat Forebrain. ICC/IF: This antibody gave a positive result in IF in the following Methanol fixed cell line: SKNSH
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab110040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionCatalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannnot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+).
Tissue specificityHighly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans.
PathwayCofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.
Sequence similaritiesBelongs to the eukaryotic NMN adenylyltransferase family.
Cellular localizationCytoplasm. Golgi apparatus.
- Information by UniProt
- C1orf15 antibody
- KIAA0479 antibody
- MGC2756 antibody
All lanes : Anti-NMNAT2 antibody (ab110040) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Rat Cortex Tissue Lysate
Lane 4 : Mouse Cortex Tissue Lysate
Lane 5 : Rat Forebrain Rat Tissue Lysate
Lane 6 : Lung (Rat) Tissue Lysate
Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Both Rat Lung tissue lysate and NIH3T3 whole cell lysate were used as negative controls for Nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2)
ab110040 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110040 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.