Key features and details
- Rabbit polyclonal to NMT1/NMT
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NMT1/NMT antibody
See all NMT1/NMT primary antibodies
DescriptionRabbit polyclonal to NMT1/NMT
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide within Human NMT1/NMT aa 50-100. The exact sequence is proprietary.
Database link: P30419
- HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
This product was previously labelled as NMT1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 57 kDa.|
|IP||Use a concentration of 2 - 10 µg/ml.|
FunctionAdds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
Tissue specificityHeart, gut, kidney, liver and placenta.
Sequence similaritiesBelongs to the NMT family.
- Information by UniProt
- Alternative, short form NMT S antibody
- EC 184.108.40.206 antibody
- Glycylpeptide N tetradecanoyltransferase 1 antibody
All lanes : Anti-NMT1/NMT antibody (ab186123) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds
Lane 1: ab186123 at 1 µg/ml staining NMT1/NMT in 293T whole cell lysate immunoprecipitated using ab186123 at 6 µg/reaction (0.5 or 1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG.
ab186123 has not yet been referenced specifically in any publications.