Key features and details
- Rabbit polyclonal to NMT1/NMT
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NMT1/NMT antibody
See all NMT1/NMT primary antibodies
DescriptionRabbit polyclonal to NMT1/NMT
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Xenopus tropicalis
- HeLa, 293T, Jurkat, TCMK-1 and NIH 3T3 whole cell lysates.
This product was previously labelled as NMT1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab186131 was affinity purified using an epitope specific to NMT1/NMT immobilized on solid support.
Our Abpromise guarantee covers the use of ab186131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 57 kDa.|
FunctionAdds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
Tissue specificityHeart, gut, kidney, liver and placenta.
Sequence similaritiesBelongs to the NMT family.
- Information by UniProt
- Alternative, short form NMT S antibody
- EC 22.214.171.124 antibody
- Glycylpeptide N tetradecanoyltransferase 1 antibody
All lanes : Anti-NMT1/NMT antibody (ab186131) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds
Detection of NMT1/NMT in immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186131 at 6 µg per reaction for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 seconds.
ab186131 has not yet been referenced specifically in any publications.