Key features and details
- Rabbit polyclonal to NMT1/NMT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NMT1/NMT antibody
See all NMT1/NMT primary antibodies
DescriptionRabbit polyclonal to NMT1/NMT
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant fragment within Human NMT1/NMT (N terminal). The exact sequence is proprietary.
Database link: P30419
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Mouse liver and brain tissues; Rat testis tissue. ICC/IF: HeLa cells.
This product was previously labelled as NMT1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 57 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionAdds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
Tissue specificityHeart, gut, kidney, liver and placenta.
Sequence similaritiesBelongs to the NMT family.
- Information by UniProt
- Alternative, short form NMT S antibody
- EC 184.108.40.206 antibody
- Glycylpeptide N tetradecanoyltransferase 1 antibody
All lanes : Anti-NMT1/NMT antibody (ab226857) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
10% SDS-PAGE gel.
Paraffin-embedded mouse liver tissue stained for NMT1/NMT with ab226857 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse brain tissue stained for NMT1/NMT with ab226857 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat testis tissue stained for NMT1/NMT with ab226857 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NMT1/NMT (green) using ab226857 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226857 has not yet been referenced specifically in any publications.