Overview

  • Product name

  • Description

    Rabbit polyclonal to NMT1/NMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human NMT1/NMT (N terminal). The exact sequence is proprietary.
    Database link: P30419

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Mouse liver and brain tissues; Rat testis tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as NMT1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
  • Tissue specificity

    Heart, gut, kidney, liver and placenta.
  • Sequence similarities

    Belongs to the NMT family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alternative, short form NMT S antibody
    • EC 2.3.1.97 antibody
    • Glycylpeptide N tetradecanoyltransferase 1 antibody
    • Glycylpeptide N-tetradecanoyltransferase 1 antibody
    • Long form, NMT L antibody
    • Myristoyl CoA:protein N myristoyltransferase 1 antibody
    • Myristoyl-CoA:protein N-myristoyltransferase 1 antibody
    • N myristoyltransferase 1 antibody
    • NMT 1 antibody
    • NMT antibody
    • nmt1 antibody
    • NMT1_HUMAN antibody
    • Peptide N myristoyltransferase 1 antibody
    • Peptide N-myristoyltransferase 1 antibody
    • Type I N myristoyltransferase antibody
    • Type I N-myristoyltransferase antibody
    see all

Images

  • All lanes : Anti-NMT1/NMT antibody (ab226857) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 57 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded mouse liver tissue stained for NMT1/NMT with ab226857 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse brain tissue stained for NMT1/NMT with ab226857 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat testis tissue stained for NMT1/NMT with ab226857 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NMT1/NMT (green) using ab226857 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

References

ab226857 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226857.
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