Product nameAnti-NMT2 antibody
See all NMT2 primary antibodies
DescriptionRabbit polyclonal to NMT2
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
- WB: Human placenta tissue lysate; K562, A431 and HepG2 whole cell lysate. IHC-P: Human testis and prostate tissue. IP: HepG2 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).|
|IP||1/200 - 1/2000.|
FunctionAdds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
Sequence similaritiesBelongs to the NMT family.
- Information by UniProt
- EC 220.127.116.11 antibody
- Glycylpeptide N tetradecanoyltransferase 2 antibody
- Glycylpeptide N-tetradecanoyltransferase 2 antibody
All lanes : Anti-NMT2 antibody (ab230028) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Paraffin-embedded human testis tissue stained for NMT2 with ab230028 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for NMT2 with ab230028 at 1/100 dilution in immunohistochemical analysis.
NMT2 was immunoprecipitated from 0.5 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate using ab230028.
Lane 1: Control IgG IP in HepG2 whole cell lysate.
Lane 2: ab230028 in HepG2 whole cell lysate.
Lane 3: NMT2 in HepG2 whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/50,000).
ab230028 has not yet been referenced specifically in any publications.