Key features and details
- Rabbit polyclonal to NMT2
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NMT2 antibody
See all NMT2 primary antibodies
DescriptionRabbit polyclonal to NMT2
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
- WB: Human placenta tissue lysate; K562, A431 and HepG2 whole cell lysate. IHC-P: Human testis and prostate tissue. IP: HepG2 whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).|
|IP||1/200 - 1/2000.|
FunctionAdds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
Sequence similaritiesBelongs to the NMT family.
- Information by UniProt
- EC 220.127.116.11 antibody
- Glycylpeptide N tetradecanoyltransferase 2 antibody
- Glycylpeptide N-tetradecanoyltransferase 2 antibody
All lanes : Anti-NMT2 antibody (ab230028) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Paraffin-embedded human testis tissue stained for NMT2 with ab230028 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for NMT2 with ab230028 at 1/100 dilution in immunohistochemical analysis.
NMT2 was immunoprecipitated from 0.5 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate using ab230028.
Lane 1: Control IgG IP in HepG2 whole cell lysate.
Lane 2: ab230028 in HepG2 whole cell lysate.
Lane 3: NMT2 in HepG2 whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/50,000).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230028 has not yet been referenced specifically in any publications.