Overview

  • Product name

  • Description

    Rabbit polyclonal to NMT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human NMT2 aa 1-120.
    Sequence:

    MAEDSESAASQQSLELDDQDTCGIDGDNEEETEHAKGSPGGYLGAKKKKK KQKRKKEKPNSGGTKSDSASDSQEIKIQQPSKNPSVPMQKLQDIQRAMEL LSACQGPARNIDEAAKHRYQ


    Database link: O60551

  • Positive control

    • WB: Human placenta tissue lysate; K562, A431 and HepG2 whole cell lysate. IHC-P: Human testis and prostate tissue. IP: HepG2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab230028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IP 1/200 - 1/2000.

Target

  • Function

    Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
  • Sequence similarities

    Belongs to the NMT family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.3.1.97 antibody
    • Glycylpeptide N tetradecanoyltransferase 2 antibody
    • Glycylpeptide N-tetradecanoyltransferase 2 antibody
    • Myristoyl CoA:protein N myristoyltransferase 2 antibody
    • Myristoyl-CoA:protein N-myristoyltransferase 2 antibody
    • N-myristoyltransferase 2 antibody
    • NMT 2 antibody
    • NMT2 antibody
    • NMT2_HUMAN antibody
    • Peptide N myristoyltransferase 2 antibody
    • Peptide N-myristoyltransferase 2 antibody
    • Type II N myristoyltransferase antibody
    • Type II N-myristoyltransferase antibody
    see all

Images

  • All lanes : Anti-NMT2 antibody (ab230028) at 1/1000 dilution

    Lane 1 : Human placenta tissue lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 57 kDa

  • Paraffin-embedded human testis tissue stained for NMT2 with ab230028 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human prostate tissue stained for NMT2 with ab230028 at 1/100 dilution in immunohistochemical analysis.

  • NMT2 was immunoprecipitated from 0.5 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate using ab230028.

    Lane 1: Control IgG IP in HepG2 whole cell lysate.

    Lane 2: ab230028 in HepG2 whole cell lysate.

    Lane 3: NMT2 in HepG2 whole cell lysate 20 µg (Input).

    For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/50,000).

References

ab230028 has not yet been referenced specifically in any publications.

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