Key features and details
- Goat polyclonal to nmt55 / p54nrb
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-nmt55 / p54nrb antibody
See all nmt55 / p54nrb primary antibodies
DescriptionGoat polyclonal to nmt55 / p54nrb
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Dog, Cynomolgus monkey
- Mouse Brain lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab50411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 3 - 6 µg/ml.|
FunctionDNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
Involvement in diseaseNote=A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.
Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
modificationsThe N-terminus is blocked.
- Information by UniProt
- 52 kDa subunit antibody
- 54 kDa nuclear RNA and DNA binding protein antibody
- 54 kDa nuclear RNA- and DNA-binding protein antibody
Anti-nmt55 / p54nrb antibody (ab50411) at 0.1 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer).
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
All lanes : Anti-nmt55 / p54nrb antibody (ab50411) at 1/2000 dilution
Lane 1 : MEF whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : Donkey anti-goat IgG HRP
Predicted band size: 55 kDa
Observed band size: 55 kDa
This image was submitted courtesy of an Abreview, please refer to Abreview for further experimental details.
ab50411 at 3.8 µg/ml staining nmt55 / p54nrb in Human spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.
ab50411 has been referenced in 4 publications.
- Bottini S et al. Post-transcriptional gene silencing mediated by microRNAs is controlled by nucleoplasmic Sfpq. Nat Commun 8:1189 (2017). PubMed: 29084942
- Rajesh C et al. The splicing-factor related protein SFPQ/PSF interacts with RAD51D and is necessary for homology-directed repair and sister chromatid cohesion. Nucleic Acids Res 39:132-45 (2011). WB ; Mouse . PubMed: 20813759
- Ong SA et al. Rasd1 Modulates the Coactivator Function of NonO in the Cyclic AMP Pathway. PLoS One 6:e24401 (2011). WB ; Human . PubMed: 21915321
- Rajesh C et al. The interaction profile of homologous recombination repair proteins RAD51C, RAD51D and XRCC2 as determined by proteomic analysis. Proteomics 9:4071-86 (2009). WB ; Mouse . PubMed: 19658102