Key features and details
- Rabbit polyclonal to NMU
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NMU antibody
See all NMU primary antibodies
DescriptionRabbit polyclonal to NMU
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human NMU (internal sequence). The exact sequence is proprietary.
Database link: P48645
- ICC/IF: A431 cells. WB: A431 and HeLa whole cell extracts.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 20 kDa.|
FunctionStimulates muscle contractions of specific regions of the gastrointestinal tract. In humans, NmU stimulates contractions of the ileum and urinary bladder.
Tissue specificityExpressed throughout the enteric nervous system with highest levels being found in the jejunum.
Sequence similaritiesBelongs to the NmU family.
- Information by UniProt
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All lanes : Anti-NMU antibody (ab229782) at 1/1000 dilution
Lane 1 : A431 (Human epidermoid carcinoma cell line) whole cell extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 20 kDa
15% SDS-PAGE gel.
A431 (Human epidermoid carcinoma cell line) cells stained for NMU (green) using ab229782 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229782 has not yet been referenced specifically in any publications.