Overview

  • Product name

    Anti-nNav1.5 antibody [4G8:1G7]
  • Description

    Mouse monoclonal [4G8:1G7] to nNav1.5
  • Host species

    Mouse
  • Specificity

    The immunogen used was 14 amino acids long and only 6 are specific for the neonatal variant therefore it is possible it may also bind the adult splice variant.
  • Tested applications

    Suitable for: ICC/IF, WB, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human nNav1.5.

Properties

Applications

Our Abpromise guarantee covers the use of ab62388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 227 kDa.
ICC Use a concentration of 10 µg/ml.
Flow Cyt Use 1-2µg for 106 cells.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

Images

  • Ab62388 (10ug/ml) staining human Nav1.5 in Nav1.5-HEK293 recombinant cell line by immunocytochemistry.

    A: Plasma membrane localisation of Nav1.5 identified by ab62388 detected by anti-mouse Alexa Fluor-488 conjugated antibody.

    B: DAP1 co-staining of cell nuclei.
  • Overlay histogram showing SHSY-5Y cells stained with ab62388 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62388, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879>) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:

  • Utrilla RG  et al. Kir2.1-Nav1.5 Channel Complexes Are Differently Regulated than Kir2.1 and Nav1.5 Channels Alone. Front Physiol 8:903 (2017). Read more (PubMed: 29184507) »
  • Guo Q  et al. A novel mutation in the SCN5A gene contributes to arrhythmogenic characteristics of early repolarization syndrome. Int J Mol Med 37:727-33 (2016). Read more (PubMed: 26820605) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Dog Cultured Cells (mammary, tumoral)
Permeabilization
No
Specification
mammary, tumoral
Fixative
Methanol

Dr. Martina Argano

Verified customer

Submitted Aug 07 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hESC-derived cardiomyocyte)
Specification
hESC-derived cardiomyocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 04 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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