Recombinant Anti-PMF-1 antibody [EPR17298] (ab199531)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17298] to PMF-1
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PMF-1 antibody [EPR17298]
See all PMF-1 primary antibodies -
Description
Rabbit monoclonal [EPR17298] to PMF-1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2 whole cell lysate Jurkat whole cell lysate HEK293 whole cell lysate, HeLa whole cell lysate, Human fetal brain tissue lysate, Human fetal kidney tissue lysate, C6 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate,
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17298 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/80.
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WB |
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
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Notes |
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IP
1/80. |
WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT. -
Tissue specificity
Highest levels of expression in heart and skeletal muscle, with significant levels expressed in kidney and liver. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore. - Information by UniProt
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Database links
- Entrez Gene: 100527963 Human
- Entrez Gene: 11243 Human
- Entrez Gene: 67037 Mouse
- Entrez Gene: 681050 Rat
- Omim: 609176 Human
- SwissProt: Q6P1K2 Human
- SwissProt: Q9CPV5 Mouse
- Unigene: 530479 Human
see all -
Alternative names
- NNF1R antibody
- PMF 1 antibody
- PMF-1 antibody
see all
Images
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All lanes : Anti-PMF-1 antibody [EPR17298] (ab199531) at 1/1000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and diluting buffer was 5% NFDM /TBST.
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All lanes : Anti-PMF-1 antibody [EPR17298] (ab199531) at 1/5000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST.
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All lanes : Anti-PMF-1 antibody [EPR17298] (ab199531) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and diluting buffer was 5% NFDM /TBST.
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PMF-1 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179466 at 1/80 (or 5 μg). Western blot was performed using ab199531 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell extract at 10ug (input)
Lane 2: HeLa whole cell extract PMF-1 immunoprecipitate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199531 in HeLa whole cell extract immunoprecipitation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab199531 has not yet been referenced specifically in any publications.