Key features and details
- Rabbit polyclonal to NNMT
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NNMT antibody
See all NNMT primary antibodies
DescriptionRabbit polyclonal to NNMT
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human NNMT aa 1-264.
MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFC LDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKK EPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLG AVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALK SSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNE GLFSLVARKLSRPL
Database link: P40261
- WB: HepG2, HeLa and A549 whole cell lysates. IHC-P: Human liver and kidney tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionCatalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
Tissue specificityPredominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.
Sequence similaritiesBelongs to the NNMT/PNMT/TEMT family.
- Information by UniProt
- EC 18.104.22.168 antibody
- Nicotinamide N methyltransferase antibody
- Nicotinamide N-methyltransferase antibody
Paraffin-embedded human kidney tissue stained for NNMT using ab223513 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-NNMT antibody (ab223513) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Paraffin-embedded human liver tissue stained for NNMT using ab223513 at 1/100 dilution in immunohistochemical analysis.
ab223513 has been referenced in 1 publication.
- Zhang X et al. Ferroptosis is governed by differential regulation of transcription in liver cancer. Redox Biol 24:101211 (2019). PubMed: 31108460