Overview

  • Product name

  • Description

    Rabbit polyclonal to NNMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human NNMT aa 1-264.
    Sequence:

    MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFC LDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKK EPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLG AVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALK SSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNE GLFSLVARKLSRPL


    Database link: P40261

  • Positive control

    • WB: HepG2, HeLa and A549 whole cell lysates. IHC-P: Human liver and kidney tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab223513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/20 - 1/200.

Target

  • Function

    Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
  • Tissue specificity

    Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.
  • Sequence similarities

    Belongs to the NNMT/PNMT/TEMT family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.1.1.1 antibody
    • Nicotinamide N methyltransferase antibody
    • Nicotinamide N-methyltransferase antibody
    • NNMT antibody
    • NNMT_HUMAN antibody
    see all

Images

  • Paraffin-embedded human kidney tissue stained for NNMT using ab223513 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-NNMT antibody (ab223513) at 1/1000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit at 1/10000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Paraffin-embedded human liver tissue stained for NNMT using ab223513 at 1/100 dilution in immunohistochemical analysis.

References

ab223513 has not yet been referenced specifically in any publications.

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