Anti-NNMT antibody (ab272669)
Key features and details
- Rabbit polyclonal to NNMT
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NNMT antibody
See all NNMT primary antibodies -
Description
Rabbit polyclonal to NNMT -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NNMT aa 150-300.
Database link: P40261 -
Positive control
- WB: U-2 OS cells. ICC: SiHa cells. IHC-P: Human liver, kidney and skin tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. -
Tissue specificity
Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas. -
Sequence similarities
Belongs to the NNMT/PNMT/TEMT family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4837 Human
- Omim: 600008 Human
- SwissProt: P40261 Human
- Unigene: 503911 Human
- Unigene: 623245 Human
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Alternative names
- EC 2.1.1.1 antibody
- Nicotinamide N methyltransferase antibody
- Nicotinamide N-methyltransferase antibody
see all
Images
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PFA fixed, Triton X-100 permeablized SiHa cells (human cervical carcinoma cell line) labeling NNMT (green) using ab272669 at 2 ug/ml in ICC/IF analysis.
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All lanes : Anti-NNMT antibody (ab272669) at 0.4 µg/ml
Lane 1 : U-2 OS cells (Human bone osteosarcoma epithelial cell line)
Lane 2 : SK-MEL-30 cells (Human cutaneous melanoma cell line)Corresponding NNMT RNA-seq data are presented for the same cell lines.
Loading control: Anti-GAPDH.
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Immunohistochemical analysis of human liver tissue labeling NNMT with ab272669 at a 1/500 dilution.
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Immunohistochemical analysis of human kidney tissue labeling NNMT with ab272669 at a 1/500 dilution.
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Immunohistochemical analysis of human pancreas tissue labeling NNMT with ab272669 at a 1/500 dilution.
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Immunohistochemical analysis of human skin tissue labeling NNMT with ab272669 at a 1/500 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272669 has not yet been referenced specifically in any publications.