Product nameAnti-NNMT antibody
See all NNMT primary antibodies
DescriptionRabbit polyclonal to NNMT
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: U-2 OS cells, human liver tissue. IF: SiHa cells. IHC-P: Human liver tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionCatalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
Tissue specificityPredominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.
Sequence similaritiesBelongs to the NNMT/PNMT/TEMT family.
- Information by UniProt
- EC 18.104.22.168 antibody
- Nicotinamide N methyltransferase antibody
- Nicotinamide N-methyltransferase antibody
Lanes 2-5 : Anti-NNMT antibody (ab272669) at 0.4 µg/ml
Lane 1 : Molecular Weight ladder
Lane 2 : RT4 cells (Human urinary bladder cancer cell line)
Lane 3 : U-251 MG cells (Human brain glioma cell line)
Lane 4 : Human plasma (IgG/HSA depleted)
Lane 5 : Human liver tissue
PFA fixed, Triton X-100 permeablized SiHa cells (human cervical carcinoma cell line) labeling NNMT (green) using ab272669 at 2 ug/ml in ICC/IF analysis.
Paraffin-embedded formalin-fixed human liver tissue stained for NNMT using ab272669 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-NNMT antibody (ab272669) at 0.4 µg/ml
Lane 1 : U-2 OS cells (Human bone osteosarcoma epithelial cell line)
Lane 2 : SK-MEL-30 cells (Human cutaneous melanoma cell line)
Corresponding NNMT RNA-seq data are presented for the same cell lines.
Loading control: Anti-GAPDH.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272669 has not yet been referenced specifically in any publications.