Overview

  • Product name
  • Description
    Rabbit polyclonal to NNMT
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC, corresponding to amino acids 1-50 of Human NNMT

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab58743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 30 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

Images

  • Anti-NNMT antibody (ab58743) at 1/2.5 dilution + HepG2 cell lysate at 10 µg with skim milk/PBS buffer at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1: 50,000 - 100,000

    Predicted band size: 30 kDa
    Observed band size: 29 kDa
    why is the actual band size different from the predicted?



    Gel concentration: 12%

References

This product has been referenced in:
See all 4 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting Abcam.

I am sorry that you are having problems with ab58743 in western blot. To be able to better assist you in troubleshooting this problem, would you be able to answer the questions below:

1 - Are you seeing any bands (if so, what size?), no bands etc?

2 - How much sample are you loading?

3 - What type of blocking agent are you using, milk, BSA etc?

4 - What primary antibody concentrations have you tried (only 1/1000) and how long do you incubate for?

The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

I look forward to your reply and helping you resolve this issue.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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