Anti-nNOS (neuronal) antibody (ab15203)
Key features and details
- Rabbit polyclonal to nNOS (neuronal)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-nNOS (neuronal) antibody
See all nNOS (neuronal) primary antibodies -
Description
Rabbit polyclonal to nNOS (neuronal) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Synthetic peptide within Mouse nNOS (neuronal) (C terminal). The exact sequence is proprietary.
Database link: P29477 -
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
Notes |
---|
IHC-P
1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
-
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
Tissue specificity
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
Domain
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
Post-translational
modificationsUbiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
Cellular localization
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
-
Database links
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 24598 Rat
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
- SwissProt: P29476 Rat
- Unigene: 654410 Human
see all -
Alternative names
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
Images
-
ab15203 staining nNOS (neuronal) in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Sections were deparaffinized, rehydrated and submitted to antigen retrieval and blocked with 5% non-fat dry milk and 0.1% Triton X-100. Samples were incubated with primary antibody (1/200 in PBS containing 2% non-fat dry milk and 0.05% Trition X-00) overnight at 4°C. A Biotin-conjugated anti-rabbit IgG (1/200 in PBS containing 1% NGS for 1 hour at room temperature) was used as the secondary antibody. Left - wild type, Right - Cre+/RTN-1C+. Magnification: 20x.
-
Human placenta tissue section stained with ab15203 by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab15203 has been referenced in 8 publications.
- Sadeghi MA et al. Chronically altered NMDAR signaling in epilepsy mediates comorbid depression. Acta Neuropathol Commun 9:53 (2021). PubMed: 33762011
- Mauriello A et al. Hypertension in kidney transplantation is associated with an early renal nerve sprouting. Nephrol Dial Transplant 32:1053-1060 (2017). PubMed: 28498963
- Tuncer AA et al. The Protective Effects of Alpha-Lipoic Acid and Coenzyme Q10 Combination on Ovarian Ischemia-Reperfusion Injury: An Experimental Study. Adv Med 2016:3415046 (2016). IHC . PubMed: 27597986
- Thuy le TT et al. Cytoglobin deficiency promotes liver cancer development from hepatosteatosis through activation of the oxidative stress pathway. Am J Pathol 185:1045-60 (2015). PubMed: 25665792
- Keles I et al. Prevention of cyclophosphamide-induced hemorrhagic cystitis by resveratrol: a comparative experimental study with mesna. Int Urol Nephrol N/A:N/A (2014). Rat . PubMed: 25248628
- Masliukov PM et al. Development of nNOS-positive neurons in the rat sensory and sympathetic ganglia. Neuroscience 256C:271-281 (2013). IHC ; Rat . PubMed: 24161722
- Fazi B et al. Characterization of gene expression induced by RTN-1C in human neuroblastoma cells and in mouse brain. Neurobiol Dis 40:634-44 (2010). WB, IHC-P, IF ; Mouse . PubMed: 20708685
- Martínez-Cerdeño V et al. Embryonic MGE precursor cells grafted into adult rat striatum integrate and ameliorate motor symptoms in 6-OHDA-lesioned rats. Cell Stem Cell 6:238-50 (2010). IHC-FoFr ; Rat . PubMed: 20207227