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Neuroscience Neurotransmission Nitric Oxide NOS
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Anti-nNOS (neuronal) antibody (ab5586)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
  • Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab5586)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
  • Western blot - Anti-nNOS (neuronal) antibody (ab5586)
  • Western blot - Anti-nNOS (neuronal) antibody (ab5586)

Key features and details

  • Rabbit polyclonal to nNOS (neuronal)
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human nNOS (neuronal) protein (ab159005)
Primary
Product image
Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-nNOS (neuronal) antibody
    See all nNOS (neuronal) primary antibodies
  • Description

    Rabbit polyclonal to nNOS (neuronal)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human nNOS (neuronal) aa 1411-1425.
    Sequence:

    NRLRSESIAFIEESK


    (Peptide available as ab5891)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Assay kits

    • Nitric Oxide Synthase Activity Assay Kit (Colorimetric) (ab211083)
    • Intracellular Nitric Oxide Synthase Detection Assay Kit (ab211085)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human nNOS (neuronal) protein (ab159005)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Mouse
Human
WB
Mouse
Rat
All applications
Rabbit
Xenopus laevis
Zebrafish
Application Abreviews Notes
ICC/IF
1/50 - 1/500.
WB
1/100 - 1/1000. Predicted molecular weight: 160 kDa.
IHC-P (1)
1/20 - 1/200.
Notes
ICC/IF
1/50 - 1/500.
WB
1/100 - 1/1000. Predicted molecular weight: 160 kDa.
IHC-P
1/20 - 1/200.

Target

  • Function

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • Tissue specificity

    Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • Sequence similarities

    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • Domain

    The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • Post-translational
    modifications

    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • Cellular localization

    Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Target information above from: UniProt accession P29475 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4842 Human
    • Entrez Gene: 18125 Mouse
    • Entrez Gene: 100009243 Rabbit
    • Entrez Gene: 24598 Rat
    • Entrez Gene: 60658 Zebrafish
    • Omim: 163731 Human
    • SwissProt: P29475 Human
    • SwissProt: Q9Z0J4 Mouse
    • SwissProt: O19132 Rabbit
    • SwissProt: P29476 Rat
    • Unigene: 654410 Human
    • Unigene: 684465 Human
    • Unigene: 684466 Human
    • Unigene: 684467 Human
    • Unigene: 442195 Mouse
    • Unigene: 44249 Mouse
    • Unigene: 10573 Rat
    see all
  • Alternative names

    • 2310005C01Rik antibody
    • BNOS antibody
    • Constitutive NOS antibody
    • EC 1.14.13.39 antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N-NOS antibody
    • NC-NOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • NNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)

    ab5586 labelling nNOS (neuronal) in the cytoplasm of Mouse heart tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab5586)
    Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab5586)

    Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (green) showing staining in the cytoplasm and membrane of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5586 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)

    ab5586 labelling nNOS (neuronal) in the cytoplasm of Human testis tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)

    ab5586 labelling nNOS (neuronal) in the cytoplasm of Human skeletal muscle (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Western blot - Anti-nNOS (neuronal) antibody (ab5586)
    Western blot - Anti-nNOS (neuronal) antibody (ab5586)
    Anti-nNOS (neuronal) antibody (ab5586) at 1/500 dilution + Mouse brain cell lysate at 25 µg

    Predicted band size: 160 kDa
    Observed band size: 160 kDa

  • Western blot - Anti-nNOS (neuronal) antibody (ab5586)
    Western blot - Anti-nNOS (neuronal) antibody (ab5586)
    Anti-nNOS (neuronal) antibody (ab5586) at 1/200 dilution + Rat brain cell lysate at 25 µg

    Predicted band size: 160 kDa
    Observed band size: 160 kDa

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab5586? Please let us know so that we can cite the reference in this datasheet.

ab5586 has been referenced in 6 publications.

  • Buniatian GH  et al. Antifibrotic Effects of Amyloid-Beta and Its Loss in Cirrhotic Liver. Cells 9:N/A (2020). PubMed: 32089540
  • Kalinichenko SG  et al. Gaseous transmitters in human retinogenesis. Acta Histochem 121:604-610 (2019). PubMed: 31113654
  • Si Z  et al. Effects of thrombolysis within 6 hours on acute cerebral infarction in an improved rat embolic middle cerebral artery occlusion model for ischaemic stroke. J Cell Mol Med 23:2468-2474 (2019). PubMed: 30697923
  • Tan C  et al. Extensive protein S-nitrosylation associated with human pancreatic ductal adenocarcinoma pathogenesis. Cell Death Dis 10:914 (2019). PubMed: 31801946
  • Xiyuan Z  et al. NO-sGC Pathway Modulates Ca2+Release and Muscle Contraction in Zebrafish Skeletal Muscle. Front Physiol 8:607 (2017). PubMed: 28878687
  • Rosa SC  et al. Nitric oxide synthase isoforms and NF-kappaB activity in normal and osteoarthritic human chondrocytes: regulation by inducible nitric oxide. Nitric Oxide 19:276-83 (2008). WB ; Human . PubMed: 18675932

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-nNOS (neuronal) antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus monkey Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Colon
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Dr. Faye Doherty

Verified customer

Submitted Aug 17 2017

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