Key features and details
- Sheep polyclonal to nNOS (neuronal)
- Suitable for: IHC-Fr
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-nNOS (neuronal) antibody
See all nNOS (neuronal) primary antibodies
DescriptionSheep polyclonal to nNOS (neuronal)
SpecificityReacts with nNOS. Cross-reactivity with iNOS or eNOS not known, but not expected.
Tested applicationsSuitable for: IHC-Frmore details
Species reactivityReacts with: Rat
General notesThis antibody is a high quality immunohistochemical reagent that has been used for studies in both the peripheral and central nervous system. It is also useful for double label studies when combined with rabbit or other primary antibodies.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/2000.
1/100 - 1/2000.
FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Tissue specificityIsoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
Sequence similaritiesBelongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain.
DomainThe PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
modificationsUbiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
Cellular localizationCell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
- Information by UniProt
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
ab6175 has been referenced in 5 publications.
- Reuss S et al. Neuroglobin Expression in the Mammalian Auditory System. Mol Neurobiol 53:1461-1477 (2016). IHC-FoFr ; Mouse . PubMed: 25636685
- Stenkamp-Strahm CM et al. Prolonged high fat diet ingestion, obesity, and type 2 diabetes symptoms correlate with phenotypic plasticity in myenteric neurons and nerve damage in the mouse duodenum. Cell Tissue Res 361:411-26 (2015). PubMed: 25722087
- Colucci M et al. Intestinal dysmotility and enteric neurochemical changes in a Parkinson's disease rat model. Auton Neurosci 169:77-86 (2012). PubMed: 22608184
- Buchwalow IB et al. Nitric oxide synthase in muscular dystrophies: a re-evaluation. Acta Neuropathol (Berl) 111:579-88 (2006). PubMed: 16718354
- Buchwalow IB et al. Compartmentalization of NO signaling cascade in skeletal muscles. Biochem Biophys Res Commun 330:615-21 (2005). PubMed: 15796928