• Product name
    Anti-nNOS (neuronal) antibody
    See all nNOS (neuronal) primary antibodies
  • Description
    Sheep polyclonal to nNOS (neuronal)
  • Host species
  • Specificity
    Reacts with nNOS. Cross-reactivity with iNOS or eNOS not known, but not expected.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Guinea pig
  • Immunogen

    Synthetic peptide:


    conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 1409-1429 of Rat NOS1 (nNOS).

  • Positive control
    • IHC: Rat brain stem tissue.
  • General notes
    This antibody is a high quality immunohistochemical reagent that has been used for studies in both the peripheral and central nervous system. It is also useful for double label studies when combined with rabbit or other primary antibodies.



Our Abpromise guarantee covers the use of ab6175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. It has been reported that the antibody can give mild background in some instances.
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr 1/100 - 1/2000.


  • Function
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • Tissue specificity
    Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • Sequence similarities
    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • Domain
    The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • Post-translational
    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • Cellular localization
    Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310005C01Rik antibody
    • BNOS antibody
    • Constitutive NOS antibody
    • EC antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N-NOS antibody
    • NC-NOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • NNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all


  • Rat brain stem tissue staining for nNOS with ab6175 (1:2000) (perfusion fixed frozen sections).


This product has been referenced in:
  • Reuss S  et al. Neuroglobin Expression in the Mammalian Auditory System. Mol Neurobiol 53:1461-1477 (2016). IHC-FoFr ; Mouse . Read more (PubMed: 25636685) »
  • Colucci M  et al. Intestinal dysmotility and enteric neurochemical changes in a Parkinson's disease rat model. Auton Neurosci 169:77-86 (2012). Read more (PubMed: 22608184) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

As for ab72428, I will need to check with the lab regarding tested tissue,localization of staining and potential cross-reactivity with eNOS and iNOS. I will letyou know what I find out. Thank you for your patience. ...

Read More

Thank you for contacting us.

We have 3 antibodies that were tested and are guaranteed (https://www.abcam.com/Abpromise) for IHC-P and rabbit samples: ab1376,ab6175 and ab72428. I would recommend trying one of thosethat were tested as they ar...

Read More

I'm sorry to hear you are experiencing problems with ab6175, I have received feedback from the laboratory that informed me that your protocol is very similar to ours and we would expect it to work, except the addition of tritonx100 in the antibody dilu...

Read More

All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity).

A positive control tissue for this antibody is the thoracic spinal cord (of rat). Pre-ganglionic sympathetic neurons stain for NOS.

We cannot provide a definitive answer to this query. However, it is unlikely that our antibody to bNOS recognizes inducible NOS.

This antibody, ab6175, is known to react with bNOS in rabbits.

Yes the antibody does cross react with mouse.

This anti-NOS antibody has not, to our knowledge, been tested in paraffin sections, but we would be surprised if this antibody did not work in paraffin. It is an antibody that is well suited to immunohistochemistry and we have found it works well for ...

Read More


Sign up