Overview

  • Product name
    Anti-nNOS (neuronal) antibody
    See all nNOS (neuronal) primary antibodies
  • Description
    Sheep polyclonal to nNOS (neuronal)
  • Host species
    Sheep
  • Specificity
    Reacts with nNOS. Cross-reactivity with iNOS or eNOS not known, but not expected.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Guinea pig
  • Immunogen

    Synthetic peptide:

    RSESIAFIEESKKDADEVFSS

    conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 1409-1429 of Rat NOS1 (nNOS).

  • Positive control
    • IHC: Rat brain stem tissue.
  • General notes
    This antibody is a high quality immunohistochemical reagent that has been used for studies in both the peripheral and central nervous system. It is also useful for double label studies when combined with rabbit or other primary antibodies.

Properties

Applications

Our Abpromise guarantee covers the use of ab6175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. It has been reported that the antibody can give mild background in some instances.
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr 1/100 - 1/2000.

Target

  • Function
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • Tissue specificity
    Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • Sequence similarities
    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • Domain
    The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • Post-translational
    modifications
    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • Cellular localization
    Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310005C01Rik antibody
    • BNOS antibody
    • Constitutive NOS antibody
    • EC 1.14.13.39 antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N-NOS antibody
    • NC-NOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • NNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

Images

  • Rat brain stem tissue staining for nNOS with ab6175 (1:2000) (perfusion fixed frozen sections).

References

This product has been referenced in:
  • Reuss S  et al. Neuroglobin Expression in the Mammalian Auditory System. Mol Neurobiol 53:1461-1477 (2016). IHC-FoFr ; Mouse . Read more (PubMed: 25636685) »
  • Colucci M  et al. Intestinal dysmotility and enteric neurochemical changes in a Parkinson's disease rat model. Auton Neurosci 169:77-86 (2012). Read more (PubMed: 22608184) »
See all 4 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer

Thank you for your reply.

As for ab72428, I will need to check with the lab regarding tested tissue,localization of staining and potential cross-reactivity with eNOS and iNOS. I will letyou know what I find out. Thank you for your patience.

As for references, we list them on the online datasheet in a tab called "Specific references" if we are aware of any. Also, customer reviews can contain testing data on new species and those would be listed in the tab "Abreviews" on the online datasheet.

For ab72428:
References: https://www.abcam.com/nNOS-neuronal-antibody-C-terminal-ab72428-references.html
Abreviews: wehave not yet received Abreviews from other customers for this antibody.

As for ab106417, a rabbit polyclonal, yes, high background due to crossreactivity of the secondary antibody (anti-rabbit) with the endogenous rabbit IgG (just as mouse-on-mouse staining)could potentially pose a problem, but there are ways to get around this.
Here is our mouse-on-mouse staining advice:
https://www.abcam.com/index.html?pageconfig=resource&rid=11465
which can be adapted to rabbit-on-rabbit staining as well.

I hope this information is of some help so far. I will be in touch with more details soon.
In the meantime, please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

We have 3 antibodies that were tested and are guaranteed (https://www.abcam.com/Abpromise) for IHC-P and rabbit samples: ab1376,ab6175 and ab72428. I would recommend trying one of thosethat were tested as they are covered under our guarantee.
We do not compare antibodies side by side, thus, I have no infomation on which of these antibodies works best in rabbit tissue. If you had unsuccessful experiences with immunogens similar to ab6175 and ab1376, I would suggest to try ab72428.

We have 3 other nNOS antibodies that are tested in IHC-P and predicted to react with rabbit (based on sequence homology) but have not yet been tested in this species:
ab79051, ab106417, ab95436

As for ab3511: This antibody was not tested in IHC-P, thus we do not know how well it would work in this application.

If you decide to try any of the 4 untestedantibodies, you can use our testing discount program. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

I'm sorry to hear you are experiencing problems with ab6175, I have received feedback from the laboratory that informed me that your protocol is very similar to ours and we would expect it to work, except the addition of tritonx100 in the antibody dilution buffer which significantly helps penetration of the antibody into the tissue. Indeed rat brain is a very good positive control and in our hands the antibody works at 1:2000 (with some background occasionally). This suggests that the antibody has been damaged during shipping or storage. If you purchased the antibody in the last 90 days I can arrange for a replacement vial or refund/credit note if you can provide me with your order details,

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Question
Answer

All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity).

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Answer

A positive control tissue for this antibody is the thoracic spinal cord (of rat). Pre-ganglionic sympathetic neurons stain for NOS.

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Answer

We cannot provide a definitive answer to this query. However, it is unlikely that our antibody to bNOS recognizes inducible NOS.

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Question
Answer

This antibody, ab6175, is known to react with bNOS in rabbits.

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Question
Answer

Yes the antibody does cross react with mouse.

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Answer

This anti-NOS antibody has not, to our knowledge, been tested in paraffin sections, but we would be surprised if this antibody did not work in paraffin. It is an antibody that is well suited to immunohistochemistry and we have found it works well for human brain (frozen sections).

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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