Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)
- Datasheet
- Specific References (13)
- Protocols
Overview
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Product nameAnti-nNOS (neuronal) (phospho S1417) antibody
See all nNOS (neuronal) primary antibodies -
DescriptionRabbit polyclonal to nNOS (neuronal) (phospho S1417)
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-FoFr, WB, IHC-Fr, ICC, IP, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Predicted to work with: Xenopus laevis, Zebrafish
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Immunogen
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab5583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 20881244 | |
| WB | Use a concentration of 2 µg/ml. Predicted molecular weight: 160 kDa.Can be blocked with Human nNOS (neuronal) (phospho S1417) peptide (ab41773). | |
| IHC-Fr | 1/500. | |
| ICC | 1/10 - 1/100. | |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | 1/10 - 1/100. |
Target
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FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
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Tissue specificityIsoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
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Sequence similaritiesBelongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
DomainThe PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
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Post-translational
modificationsUbiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
Cellular localizationCell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
- Information by UniProt
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Database links
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 100009243 Rabbit
- Entrez Gene: 24598 Rat
- Entrez Gene: 60658 Zebrafish
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
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Alternative names
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)This image is courtesy of an Abreview submitted by Jose Donatoab5583 staining nNOS (phospho S1417) in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formalin, permeabilized with 0.25% Triton X-100 and blocked with 3% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/500 in PBS + Triton X-10 + normal donkey serum) for 48 hours at 4°C. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/10000) was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (phospho S1417) (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells treated with 100 ng/ml nocodazole (left) and untreated SH-SY5Y cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5583 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Protocols
References
This product has been referenced in:
- Jean A et al. Revisiting medial preoptic area plasticity induced in male mice by sexual experience. Sci Rep 7:17846 (2017). IHC-P ; Mouse . Read more (PubMed: 29259324) »
- Sastre E et al. Decompensated liver cirrhosis and neural regulation of mesenteric vascular tone in rats: role of sympathetic, nitrergic and sensory innervations. Sci Rep 6:31076 (2016). WB ; Rat . Read more (PubMed: 27484028) »
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