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Neuroscience Neurotransmission Nitric Oxide NOS
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Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)

  • Datasheet
Reviews (6)Q&A (7)References (18)

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Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)

    Key features and details

    • Rabbit polyclonal to nNOS (neuronal) (phospho S1417)
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

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    Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Protein
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    Recombinant Human nNOS (neuronal) protein (ab159005)
    Primary
    Product image
    Anti-nNOS (neuronal) (phospho S847) antibody (ab16650)

    View more associated products

    Overview

    • Product name

      Anti-nNOS (neuronal) (phospho S1417) antibody
      See all nNOS (neuronal) primary antibodies
    • Description

      Rabbit polyclonal to nNOS (neuronal) (phospho S1417)
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      ICC/IF
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human nNOS (neuronal) aa 1411-1425 (phospho S1417).
      Sequence:

      CNRLRSE(pS)IAFIEESK


      Database link: P29475
      (Peptide available as ab41773)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Nitric Oxide
      • NOS
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Assay kits

      • Nitric Oxide Synthase Activity Assay Kit (Colorimetric) (ab211083)
      • Intracellular Nitric Oxide Synthase Detection Assay Kit (ab211085)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human nNOS (neuronal) protein (ab159005)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab5583 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Human
    All applications
    Xenopus laevis
    Zebrafish
    Application Abreviews Notes
    ICC/IF
    1/10 - 1/100.
    Notes
    ICC/IF
    1/10 - 1/100.

    Target

    • Function

      Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
    • Tissue specificity

      Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
    • Sequence similarities

      Belongs to the NOS family.
      Contains 1 FAD-binding FR-type domain.
      Contains 1 flavodoxin-like domain.
      Contains 1 PDZ (DHR) domain.
    • Domain

      The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
    • Post-translational
      modifications

      Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
    • Cellular localization

      Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
    • Target information above from: UniProt accession P29475 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4842 Human
      • Entrez Gene: 60658 Zebrafish
      • Omim: 163731 Human
      • SwissProt: P29475 Human
      • Unigene: 654410 Human
      • Unigene: 684465 Human
      • Unigene: 684466 Human
      • Unigene: 684467 Human
      • Alternative names

        • 2310005C01Rik antibody
        • BNOS antibody
        • Constitutive NOS antibody
        • EC 1.14.13.39 antibody
        • IHPS 1 antibody
        • IHPS1 antibody
        • N-NOS antibody
        • NC-NOS antibody
        • neuronal Nitric Oxide Synthase antibody
        • Neuronal NOS antibody
        • Nitric oxide synthase , neuronal, included antibody
        • Nitric oxide synthase 1 (neuronal) antibody
        • Nitric oxide synthase 1 antibody
        • Nitric oxide synthase, brain antibody
        • Nitric oxide synthase, penile neuronal, included antibody
        • NNOS antibody
        • NO antibody
        • NOS 1 antibody
        • NOS antibody
        • NOS type I antibody
        • NOS-I antibody
        • NOS1 antibody
        • NOS1_HUMAN antibody
        • Peptidyl-cysteine S-nitrosylase NOS1 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)
        Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)

        Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (phospho S1417) (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells treated with 100 ng/ml nocodazole (left) and untreated SH-SY5Y cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5583 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (18)

      Publishing research using ab5583? Please let us know so that we can cite the reference in this datasheet.

      ab5583 has been referenced in 18 publications.

      • Ho CY  et al. CX3CR1-microglia mediates neuroinflammation and blood pressure regulation in the nucleus tractus solitarii of fructose-induced hypertensive rats. J Neuroinflammation 17:185 (2020). PubMed: 32532282
      • Tomiga Y  et al. Anxiety-like behaviors and hippocampal nNOS in response to diet-induced obesity combined with exercise. J Physiol Sci 69:711-722 (2019). PubMed: 31124076
      • Wang Z  et al. Quantitative phosphoproteomic analysis of the molecular substrates of sleep need. Nature 558:435-439 (2018). PubMed: 29899451
      • Arce C  et al. Activation of a1A -adrenoceptors desensitizes the rat aorta response to phenylephrine through a neuronal NOS pathway, a mechanism lost with ageing. Br J Pharmacol 174:2015-2030 (2017). PubMed: 28369791
      • Jean A  et al. Revisiting medial preoptic area plasticity induced in male mice by sexual experience. Sci Rep 7:17846 (2017). IHC-P ; Mouse . PubMed: 29259324
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 13 Abreviews or Q&A

      Western blot abreview for Anti-nNOS (neuronal) (phospho S1417) antibody

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Apteronotus leptorhynchus Tissue lysate - whole (Brain)
      Loading amount
      50 µg
      Specification
      Brain
      Gel Running Conditions
      Reduced Denaturing (4-15%)
      Blocking step
      Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 31 2013

      Question

      Problem: no band

      Sorry for the delay.Here are the needed information:

      1) What was the other sample type used (apart from mouse brain)? DRG's

      2) How much protein was loaded? 40ug

      3) What was the lysis buffer?

      4) What was the blocking step? 5% milk or 5% BSA

      5) What dilution was used for the primary antibody? How long was the incubation? 1:1000 for overnight

      6) What percent gel was used? 10%

      Read More

      Abcam community

      Verified customer

      Asked on Nov 13 2012

      Answer

      Thank you for this information. Based on your protocol I think increasing the amount of primary antibody you use would improve your result. We recommend using 2 ug/ml, which would be 1/500. I would try using this (or even 1/250) overnight at 4 degrees. The GAPDH will be much more highly expressed than the phospho nNOS so I am not surprised that it is showing a good band. Please let me know if you are still having difficulty.

      Read More

      Abcam Scientific Support

      Answered on Nov 13 2012

      Western blot abreview for Anti-nNOS (neuronal) (phospho S1417) antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (Not Given)
      Loading amount
      30 µg
      Specification
      Not Given
      Gel Running Conditions
      Reduced Denaturing (4-15%)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 04 2012

      Question

      Thanks for the information. We purchase on Fridays, so we would purchase ab5583 this Friday. Would I then send you some kind of confirmation to be sure that I will get the code?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 15 2012

      Answer

      I am very pleased to hear you would like to use ab5583 in WB and send us an image of your results.

      This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview with the image for WB and include this code in the Additional Comments section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

      For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
      1) Call to place your order and mention the code to our customer service department;
      2) Include the code in your fax order;
      3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

      If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Aug 15 2012

      Question

      I have a quick question: if I submit an a review with picture for ab90443, having already purchased that antibody, can I still take advantage od this deal?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 15 2012

      Answer

      Thank you for your reply.


      If you have purchased ab90443 within the last month and are able to submit data in ICC, I am able to issue a code retroactively. However, you will not be able to use this code to obtain ab5583 to test it. You must purchase the antibody to be tested in order to receive a valid code.


      I hope this information is helpful. Please let me know and I will issue the code for ab90443 and/or ab5583.

      Read More

      Abcam Scientific Support

      Answered on Aug 15 2012

      Question

      I would like to test ab5583 and submit a WB image. Also, I would be interested in testing ab90443 for ICC.

      Read More

      Abcam community

      Verified customer

      Asked on Aug 14 2012

      Answer

      Thank you very much for your interest in ab5583 and ab90443.

      We do not currently have an image of ab5583 in WB and ab90443 has not been tested in ICC. Therefore, I can offer a discount off a future purchase if you buyab5583 and/or ab90443 now, use it in WB, ICC and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody.

      It doesn't matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab5583, ab90443 in WB, ICC. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

      2. Purchase ab5583, ab90443either by phone, fax, or online (www.abcam.com).

      3. Use it in WB, ICC.

      4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated!

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Aug 14 2012

      Question

      ab5583 not working in WB with human and mouse brain samples.
      One previousvial has worked well. Tried this new vial once, it has not worked.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 18 2012

      Answer

      Thank you for your telephone enquiry this afternoon. I am sorry to hear you have had difficulty obtaining satisfactory results from the current vial of this antibody.

      I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

      As discussed on the telephone, I would like to reassure you that this antibody is tested and covered by our guarantee for WB and mouse. Before deciding how to proceed, I would like to investigate this particular case further for you, and also obtain some further information for our quality records.

      In order to do this, I have enclosed a questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily. I would also appreciate if you are able to provide images, including molecular weight markers, from the vials that have and have not worked, this will be helpful for our investigations and assessment of the results.

      In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

      Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.

      Order Details
      Antibody code:
      Problem Choose: Non-specific band Multiple bands No signal or weak signal High background
      Lot number
      Purchase order number or preferably Abcam order number:
      General Information Antibody storage conditions (temperature/reconstitution etc)
      Description of the problem (high background, wrong band size, more bands, no band etc.)
      Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)
      Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)
      Amount of protein loaded
      Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)
      Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)
      Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
      Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
      Detection method (ECL, ECLPlus etc.)
      Positive and negative controls used (please specify)
      Optimization attempts (problem solving) How many times have you tried the Western?
      Have you run a "No Primary" control? Yes No
      Do you obtain the same results every time? Yes No e.g. are the background bands always in the same place?
      What steps have you altered?
      Additional Notes:
      Image: We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to assess the results.

      Read More

      Abcam Scientific Support

      Answered on Jul 18 2012

      Immunohistochemistry (Frozen sections) abreview for Anti-nNOS (neuronal) (phospho S1417) antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Apteronotus leptorhynchus Tissue sections (Brain)
      Specification
      Brain
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.3% Triton X-100
      Blocking step
      (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
      Read More

      Dr. Ruxandra Sirbulescu

      Verified customer

      Submitted Nov 24 2011

      Western blot abreview for Anti-nNOS (neuronal) (phospho S1417) antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (Penile Tissue)
      Loading amount
      60 µg
      Specification
      Penile Tissue
      Gel Running Conditions
      Reduced Denaturing (4-15% mini gel)
      Blocking step
      Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More

      Miss. Gwen Lagoda

      Verified customer

      Submitted Nov 18 2011

      Question

      I would like to test ab16650 and ab5583 in IHC using electric fish samples.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 09 2011

      Answer

      I am very pleased to hear you would like to accept our offer and test ab16650 and ab5583 in electric fish. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for electric fish and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Nov 09 2011

      1-10 of 13 Abreviews or Q&A

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