• Product name
    Anti-NNT antibody [8B4BB10]
    See all NNT primary antibodies
  • Description
    Mouse monoclonal [8B4BB10] to NNT
  • Host species
  • Tested applications
    Suitable for: IP, ICC/IF, In-Cell ELISA, IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Human heart mitochondria

  • Positive control
    • Normal Human skin fibroblasts (strain HDFn); Human cerebellum tissue; mitochondria from Human liver, Bovine heart, Rat liver and Mouse liver; HepG2 and HL-60 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab110352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml. 0.4 µg/well
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 114 kDa.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.
  • Sequence similarities
    In the N-terminal section; belongs to the AlaDH/PNT family.
    In the C-terminal section; belongs to the PNT beta subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCCD4 antibody
    • mitochondrial antibody
    • NAD(P) transhydrogenase antibody
    • NAD(P) transhydrogenase mitochondrial antibody
    • Nicotinamide nucleotide transhydrogenase antibody
    • NNT antibody
    • NNTM antibody
    • NNTM_HUMAN antibody
    • Pyridine nucleotide transhydrogenase antibody
    see all


  • HL-60 cells were stained with 1 µg/mL NNT antibody (ab110352) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Mitochondrial localization of NNT in cultured, normal Human skin fibroblasts (strain HDFn) labeled with anti NNT (ab110352) as the primary antibody and an AlexaFluor® 594 goat anti-mouse IgG1 secondary antibody.
  • NNT immunohistochemistry in Human cerebellum visualized with ab110352 at 1/100. NNT immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
  • Nicotinamide nucleotide transhydrogenase (NNT) immunocaptured from Human liver mitochondria (lane 1), bovine heart mitochondria (lane 2), rat liver mitochondria (lane 3), mouse liver mitochondria (lane 4), and HepG2 cells (lane 5), using ab110352.
  • Anti-NNT antibody [8B4BB10] (ab110352) at 0.5 µg/ml + HepG2 whole cell lysate

    Predicted band size: 114 kDa

    anti-Complex II 30kDa subunit has also been labeled as a control.


This product has been referenced in:
  • Agarwal AR  et al. Short-term cigarette smoke exposure induces reversible changes in energy metabolism and cellular redox status independent of inflammatory responses in mouse lungs. Am J Physiol Lung Cell Mol Physiol 303:L889-98 (2012). WB ; Mouse . Read more (PubMed: 23064950) »

See 1 Publication for this product

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence
African green monkey Cell (COS-1)
Yes - 0.2% TritonX-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10)
Mouse Tissue lysate - other (Lung mitochondrion)
Lung mitochondrion
Blocking step
Casein-TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Amit Agarwal

Verified customer

Submitted Jul 18 2013

Thank you for following up on this query.

The staining displayed on the datasheet of the Anti-NNT antibody [8B4BB10] is not from an Abreview but from testing we have commissioned ourselves.

This was performed on human cerebellum tis...

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Thank you for contacting us.

I have had the opportunity to speak with the lab regarding to the antibody ab110352 [8B4BB10], we only tested human whole cell lysate (HepG2) not mouse cell lysate. This antibody has low affinity to mouse NNT b...

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I am sorry that you still did not receive a signal with the original antibody when using the positive control that I sent. I have processed you request to receive a new vial of ab110352 and you should have it by Friday of this week. I am sorry ...

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Thank you for confirming these details and for your cooperation. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order n...

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Thank you for contacting us and reporting this problem. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. The information provided by you will also allow us to investigate this case i...

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