Overview

  • Product name

    Anti-NNT antibody [8B4BB10] (HRP)
    See all NNT primary antibodies
  • Description

    Mouse monoclonal [8B4BB10] to NNT (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Tissue, cells or virus corresponding to Human NNT.

  • Positive control

    • WB: HepG2 whole cell lysate.
  • General notes

     

     

Properties

Applications

Our Abpromise guarantee covers the use of ab198312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).

Target

  • Function

    The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.
  • Sequence similarities

    In the N-terminal section; belongs to the AlaDH/PNT family.
    In the C-terminal section; belongs to the PNT beta subunit family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GCCD4 antibody
    • mitochondrial antibody
    • NAD(P) transhydrogenase antibody
    • NAD(P) transhydrogenase mitochondrial antibody
    • Nicotinamide nucleotide transhydrogenase antibody
    • NNT antibody
    • NNTM antibody
    • NNTM_HUMAN antibody
    • Pyridine nucleotide transhydrogenase antibody
    see all

Images

  • Anti-NNT antibody [8B4BB10] (HRP) (ab198312) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 114 kDa
    Observed band size: 114 kDa


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198312 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab198312 has not yet been referenced specifically in any publications.

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